caa a22 4500 397567782 CHVBK 20180308164832.0 cr unu---uuuuu 161202e199603 xx s 000 0 eng 10.3382/ps.0750393 doi (NATIONALLICENCE)oxford-10.3382/ps.0750393 OSCAR T. P. Prolonged In Vitro Exposure of Broiler Adipocytes to Somatostatin Enhances Lipolysis and Induces Desensitization of Antilipolysis [Elektronische Daten] [T. P. OSCAR] Adipocytes isolated from abdominal fat of female broilers and maintained in primary culture were used to characterize acute and chronic effects of somatostatin (SRIF) on lipolysis and antilipolysis and to determine whether desensitization and cross-regulation phenomena are involved in the regulation of adipocyte metabolism by SRIF and glucagon. Acute exposure of adipocytes to SRIF resulted in a dose-dependent inhibition of basal and glucagon-stimulated lipolysis. The potency and extent of lipolysis inhibition by SRIF were inversely related to the dose of glucagon used to stimulate lipolysis. Preincubation of adipocytes with SRIF induced a dose-response and time-dependent increase in the set-point of lipolysis. Adipocyte sensitivity to glucagon was not altered by SRIF pretreatment. In contrast, preincubation with SRIF reduced adipocyte sensitivity and maximal responsiveness to SRIF. The pattern and extent of antilipolysis attenuation were dependent on the dose and time of preincubation with SRIF as well as on the concentration of glucagon used to acutely stimulate lipolysis. In two experiments, the attenuation of antilipolysis induced by SRIF pretreatment was observed in the absence of enhanced lipolysis, whereas in one experiment enhanced lipolysis was observed in the absence of attenuated antilipolysis. These results indicated that persistent activation of antilipolysis by SRIF increased the set-point of lipolysis by sensitizing (i.e., cross-regulating) lipolysis and by desensitizing antilipolysis. © 1996 Poultry Science Association, Inc. Physiology and Reproduction nationallicence adipocyte nationallicence broiler nationallicence glucagon nationallicence lipolysis nationallicence somatostatin nationallicence Poultry Science Oxford University Press 75/3(1996-03), 393-401 0032-5791 75:3<393 1996 75 ps https://doi.org/10.3382/ps.0750393 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.3382/ps.0750393 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- OSCAR T. P. NATIONALLICENCE 773 0- Poultry Science Oxford University Press 75/3(1996-03), 393-401 0032-5791 75:3<393 1996 75 ps Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford