caa a22 4500 397567839 CHVBK 20180308164832.0 cr unu---uuuuu 161202e199605 xx s 000 0 eng 10.3382/ps.0750642 doi (NATIONALLICENCE)oxford-10.3382/ps.0750642 Mapping Functional Chicken Genes: An Alternative Approach [Elektronische Daten] [EUGENE J. SMITH, HANS H. CHENG, ROGER L. VALLEJO] Functional genes were selected for linkage analysis mapping using the East Lansing (EL) reference population {[Jungle Fowl (JF) × White Leghorn (WL)] × WL}. The approach used was based on the identification of DNA sequence polymorphisms in the introns of those genes found in JF and WL. Deoxyribonucleic acid sequence analysis revealed single base substitutions in introns of six Type I marker genes: adenylate kinase 1 (AK1), aldolase B (ALDOB), a lysosomal membrane protein gene (LAMP1), vitellogenin 2 (VTG2), apolipoprotein A1 (APOA1), and creatine kinase B (CKB). Transitions or transversions were found in introns of AK1, ALDOB, LAMP1, VTG2, APOA1, and CKB. A transversion in the intron of the JF allele of AK1 generated a unique BspHI cleavage site. The design of polymerase chain reaction (PCR) primers based on the site of base substitution led to the specific amplification of the JF allele in the remaining five genes. A size polymorphism in the PCR production derived from iron response element binding protein (IREBP) distinguished the JF from the WL allele. Linkage analysis of the EL reference population revealed that these candidate genes were located in the following EL linkage groups (E) or chromosomes (Chrom) of the chicken genome: AK1 (E41), VTG2 (E43), APOA1 (E49), CKB (E07), LAMP1 (E01), ALDOB (Chrom Z), and IREBP (Chrom Z). Provided that a base substitution can be found in the parents of the reference population, this PCR-based approach can be used to map any cloned candidate gene. This approach will lead to further information on synteny of the chicken genome with cognate genes of mammalian species. © 1996 Poultry Science Association, Inc. Molecular Biology nationallicence polymorphisms nationallicence polymerase chain reaction nationallicence mismatched primers nationallicence genetic mapping nationallicence SMITH EUGENE J. aut CHENG HANS H. aut VALLEJO ROGER L. aut Poultry Science Oxford University Press 75/5(1996-05), 642-647 0032-5791 75:5<642 1996 75 ps https://doi.org/10.3382/ps.0750642 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.3382/ps.0750642 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- SMITH EUGENE J. aut NATIONALLICENCE 700 1- CHENG HANS H. aut NATIONALLICENCE 700 1- VALLEJO ROGER L. aut NATIONALLICENCE 773 0- Poultry Science Oxford University Press 75/5(1996-05), 642-647 0032-5791 75:5<642 1996 75 ps Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford