caa a22 4500 397567901 CHVBK 20180308164833.0 cr unu---uuuuu 161202e199607 xx s 000 0 eng 10.3382/ps.0750904 doi (NATIONALLICENCE)oxford-10.3382/ps.0750904 Microsatellite Polymorphism in Commercial Broiler and Layer Lines Estimated Using Pooled Blood Samples [Elektronische Daten] [RICHARD P.M.A. CROOIJMANS, AB F. GROEN, ANTHONY J. A. VAN KAMPEN, SIJNE VAN DER BEEK, JAN J. VAN DER POEL, MARTIEN A. M. GROENEN] For 17 microsatellite markers, allele frequencies were determined in nine highly selected commercial broiler and six highly selected commercial layer lines using pooled blood samples from 60 animals. The average number of marker alleles was 5.8 over all lines, 5.2 over broiler lines, and 3.0 over layer lines. The average number of marker alleles within a line was 2.9, 3.6, and 2.0 for all, broiler, and layer lines, respectively. Over all 15 lines, the average percentage of heterozygosity was 42, whereas the heterozygosity in the broiler lines was 53% and in the layer lines only 27%. In broiler lines, 50% of the marker-line combinations showed a heterozygosity above 60%, whereas this was only 5% in layer lines. Estimation of allele frequencies with microsatellite markers was first assessed in pooled and individual samples before usage in the commercial lines. Allele frequencies for 19 microsatellite markers were estimated in chicken pooled blood samples and compared with allele frequencies from individual typed animals. Similar results were obtained when pooled blood samples (heterozygosity of 35.3%) or individual typed animals (heterozygosity of 34.2%) were used. The method to determine allele frequencies using pooled blood samples is faster, cheaper, and as reliable and repeatable as determining allele frequencies using individual typings. © 1996 Poultry Science Association, Inc. Molecular Biology nationallicence chicken nationallicence microsatellite markers nationallicence pooled blood samples nationallicence heterozygosity nationallicence population screening nationallicence CROOIJMANS RICHARD P.M.A. aut GROEN AB F. aut VAN KAMPEN ANTHONY J. A. aut VAN DER BEEK SIJNE aut VAN DER POEL JAN J. aut GROENEN MARTIEN A. M. aut Poultry Science Oxford University Press 75/7(1996-07), 904-909 0032-5791 75:7<904 1996 75 ps https://doi.org/10.3382/ps.0750904 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.3382/ps.0750904 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- CROOIJMANS RICHARD P.M.A. aut NATIONALLICENCE 700 1- GROEN AB F. aut NATIONALLICENCE 700 1- VAN KAMPEN ANTHONY J. A. aut NATIONALLICENCE 700 1- VAN DER BEEK SIJNE aut NATIONALLICENCE 700 1- VAN DER POEL JAN J. aut NATIONALLICENCE 700 1- GROENEN MARTIEN A. M. aut NATIONALLICENCE 773 0- Poultry Science Oxford University Press 75/7(1996-07), 904-909 0032-5791 75:7<904 1996 75 ps Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford