caa a22 4500 397567944 CHVBK 20180308164833.0 cr unu---uuuuu 161202e199601 xx s 000 0 eng 10.3382/ps.0750006 doi (NATIONALLICENCE)oxford-10.3382/ps.0750006 Gene Mapping by Chromosome Microdissection and Microisolation in the Chicken [Elektronische Daten] [E. M. SHAW, J. S. OTIS, S. J. LAMONT, K. S. GUISE, R. N. SHOFFNER] A chromosome microdissection and microisolation technique in combination with filter hybridization was developed for chromosomal localization of cloned chicken genes. The DNA was obtained from microdissected chromosome regions of metaphase spreads. Dissected DNA was amplified by polymerase chain reaction (PCR). The chicken MHC gene located on the nucleolar chromosome and β-actin gene located on chromosome 2q were chosen as tests for the procedure and then detected by dot blot analysis using amplified chromosomal DNA probed with biotinylated DNA. The study establishes the technique of using chromosome microdissection and microisolation for localization of cloned genes as a complementary or alternative approach to both in situ DNA/chromosome hybridization and fluorescent in situ hybridization. © 1996 Poultry Science Association, Inc. Breeding and Genetics nationallicence chromosome microdissection nationallicence chicken nationallicence chromosome nationallicence β-actin nationallicence major histocompatibility complex nationallicence SHAW E. M. aut OTIS J. S. aut LAMONT S. J. aut GUISE K. S. aut SHOFFNER R. N. 3To whom correspondence should be addressed. aut Poultry Science Oxford University Press 75/1(1996-01), 6-12 0032-5791 75:1<6 1996 75 ps https://doi.org/10.3382/ps.0750006 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.3382/ps.0750006 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- SHAW E. M. aut NATIONALLICENCE 700 1- OTIS J. S. aut NATIONALLICENCE 700 1- LAMONT S. J. aut NATIONALLICENCE 700 1- GUISE K. S. aut NATIONALLICENCE 700 1- SHOFFNER R. N. 3To whom correspondence should be addressed aut NATIONALLICENCE 773 0- Poultry Science Oxford University Press 75/1(1996-01), 6-12 0032-5791 75:1<6 1996 75 ps Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford