caa a22 4500 397568207 CHVBK 20180308164833.0 cr unu---uuuuu 161202e199606 xx s 000 0 eng 10.3382/ps.0750785 doi (NATIONALLICENCE)oxford-10.3382/ps.0750785 A New Method for Rapid Determination of Sperm Concentration in Turkey Semen [Elektronische Daten] [ANN M. DONOGHUE, DOUG THISTLETHWAITE, DAN J. DONOGHUE, JOHN D. KIRBY] The routine determination of sperm concentration not only provides a measure of semen quality but also supplies a means of delivering precise sperm numbers in modern artificial insemination (AI) programs. Methods for estimating sperm numbers in laboratory settings (e.g., hemacytometer) are not practical for use in breeding facilities. A microprocessor-controlled semen analyzer (Densimeter, Model 534-B-Mod1, Animal Reproductive Systems) is commercially available for evaluating stallion semen. We calibrated and modified the semen analyzer to determine sperm concentration in turkey semen and to provide information on the required dilution for a constant AI dose. To calibrate the semen analyzer for turkeys, six samples of pooled semen (10 toms per sample) were collected by abdominal massage. A dilution curve of undiluted and 1:0.5, 1:1, 1:1.5, 1:2, 1:3, 1:5, and 1:10 diluted semen was established using Beltsville Poultry Semen Extender. Sperm concentration in diluted samples was determined by hemacytometer counts. The hemacytometer counts were used to develop an equation for estimating sperm concentration from the semen analyzer's absorbance readings and the equation programmed into the instrument. The newly developed program on the semen analyzer was subsequently validated by comparing instrument values for sperm concentrations with concurrent hemacytometer counts, which compared well to concentrations determined by a previously calibrated photoelectric colorimeter (Klett). Correlation coefficients between the semen analyzer and hemacytometer counts and the Klett and hemacytometer counts were r = 0.996 and r = 0.992, respectively (P < 0.001). The semen analyzer method was accurate and precise and could be beneficial to commercial turkey AI programs because it is easy to use, requires limited technological skills, and provides results for determining sperm concentration and AI dose in ~1 min. © 1996 Poultry Science Association, Inc. Physiology and Reproduction nationallicence sperm concentration nationallicence turkey nationallicence hemacytometer nationallicence photometer nationallicence DONOGHUE ANN M. Germplasm and Gamete Physiology Laboratory, Agricultural Research Service, USDA, Beltsville, Maryland 20705 aut THISTLETHWAITE DOUG Animal Reproduction Systems, Chino, California 91710 aut DONOGHUE DAN J. FDA, Pharmacology-Biochemistry Branch, Beltsville, Maryland 20705 and aut KIRBY JOHN D. Department of Poultry Science, University of Arkansas, Fayetteville, Arkansas 72701 aut Poultry Science Oxford University Press 75/6(1996-06), 785-789 0032-5791 75:6<785 1996 75 ps https://doi.org/10.3382/ps.0750785 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.3382/ps.0750785 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- DONOGHUE ANN M. Germplasm and Gamete Physiology Laboratory, Agricultural Research Service, USDA, Beltsville, Maryland 20705 aut NATIONALLICENCE 700 1- THISTLETHWAITE DOUG Animal Reproduction Systems, Chino, California 91710 aut NATIONALLICENCE 700 1- DONOGHUE DAN J. FDA, Pharmacology-Biochemistry Branch, Beltsville, Maryland 20705 and aut NATIONALLICENCE 700 1- KIRBY JOHN D. Department of Poultry Science, University of Arkansas, Fayetteville, Arkansas 72701 aut NATIONALLICENCE 773 0- Poultry Science Oxford University Press 75/6(1996-06), 785-789 0032-5791 75:6<785 1996 75 ps Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford