caa a22 4500 397568215 CHVBK 20180308164833.0 cr unu---uuuuu 161202e199612 xx s 000 0 eng 10.3382/ps.0751546 doi (NATIONALLICENCE)oxford-10.3382/ps.0751546 The Male Contribution to Broiler Breeder Heat-Induced Infertility as Determined by Sperm-Egg Penetration and Sperm Storage Within the Hen's Oviduct [Elektronische Daten] [CHRISTOPHER D. MCDANIEL, R. KEITH BRAMWELL, BIRKETT HOWARTH] The purpose of the present study was to define the role of the male broiler breeder in heat-induced infertility. Seventy-two Arbor Acres roosters were individually caged at 21 wk of age and divided equally among three heated (H) and three control (C) temperature chambers. Control temperature chambers were held at 21 C. After an 8-wk pretreatment period (20 C), an 8-wk treatment period was conducted in which the temperature in all three of the H chambers was varied from week to week according to the following schedule: Week 1, 27 C; Week 2 through Week 4, 32 C; and Week 5 through Week 8, 21 C. On a weekly basis, semen was pooled by room and inseminated into 12 groups of 10 hens each (2 groups per room). During the 1st wk when males were maintained at 27 C for 12 h, in vivo sperm-egg penetration was reduced by 48% as compared to data obtained when males were maintained at 21 C. Fertility, in vivo sperm-egg penetration, and uterovaginal sperm storage were decreased when semen from males exposed to 32 C was used to inseminate hens as compared to insemination with semen from C males. However, during this same period, the ability of sperm to bind to and penetrate the egg, as determined by in vitro sperm-egg penetration, was similar between sperm from C and H males. After lowering the temperature in the H chambers back to 21 C, in vivo sperm-egg penetration as a result of insemination with semen from H males was analogous to results obtained when C males were used for insemination. Immediately after decreasing the temperature in the H chambers, fertilization of eggs by sperm from H males increased to a level similar to that obtained when eggs were fertilized by sperm from C males but then declined again during the later weeks. © 1996 Poultry Science Association, Inc. Physiology and Reproduction nationallicence sperm-egg penetration nationallicence oviductal sperm storage nationallicence fertility nationallicence heat stress nationallicence broiler breeder nationallicence MCDANIEL CHRISTOPHER D. aut BRAMWELL R. KEITH aut HOWARTH BIRKETT 4To whom correspondence should be addressed. aut Poultry Science Oxford University Press 75/12(1996-12), 1546-1554 0032-5791 75:12<1546 1996 75 ps https://doi.org/10.3382/ps.0751546 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.3382/ps.0751546 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- MCDANIEL CHRISTOPHER D. aut NATIONALLICENCE 700 1- BRAMWELL R. KEITH aut NATIONALLICENCE 700 1- HOWARTH BIRKETT 4To whom correspondence should be addressed aut NATIONALLICENCE 773 0- Poultry Science Oxford University Press 75/12(1996-12), 1546-1554 0032-5791 75:12<1546 1996 75 ps Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford