caa a22 4500 397568363 CHVBK 20180308164834.0 cr unu---uuuuu 161202e199604 xx s 000 0 eng 10.3382/ps.0750439 doi (NATIONALLICENCE)oxford-10.3382/ps.0750439 Measurement of Genetic Parameters Within and Between Turkey Lines Using DNA Fingerprinting [Elektronische Daten] [J. ZHU, K. E. NESTOR, R. A. PATTERSON, D. J. JACKWOOD, D. A. EMMERSON] An experiment was conducted to estimate genetic parameters in six experimental and five commercial primary breeding turkey lines using DNA fingerprinting. Eighteen individual DNA samples per line were digested with an HaeIII restriction enzyme and hybridized with Jeffreys' 33.6 probe. The DNA fingerprints were analyzed with computer programs to measure band sharing (BS) and band frequencies. Within lines, BS ranged from 0.39 to 0.62 and reflected the history of the experimental lines. Among lines, BS ranged from 0.21 to 0.33 with an average of 0.26. The BS among the experimental lines reflected known relationships. All lines were subdivided based on indices of population subdivision. About 26 hypervariable loci were estimated from band frequencies. Average heterozygosity and genetic variability estimated from band frequencies were significantly different among lines and displayed a result very similar to the BS among lines. Genetic distance indices among lines were also significantly different and reflected known relationships between the experimental lines. The experimental selected lines displayed lower genetic diversity than did the other lines. The parameters measuring genetic diversity within lines had higher correlation coefficients among them than did the parameters between lines. The computer program used in this study made DNA fingerprinting easier to use in population analysis. © 1996 Poultry Science Association, Inc. Breeding and Genetics nationallicence DNA fingerprinting nationallicence computer analysis nationallicence genetic diversity nationallicence genetic distance nationallicence turkey lines nationallicence ZHU J. Department of Animal Sciences and aut NESTOR K. E. Department of Animal Sciences and aut PATTERSON R. A. Department of Animal Sciences and aut JACKWOOD D. J. Food Animal Health Research Program, The Ohio State University, Ohio Agricultural Research and Development Center, Wooster, Ohio 44691 and aut EMMERSON D. A. Hybrid Turkeys Inc., Kitchener, Ontario, Canada, N2B 3E9 aut Poultry Science Oxford University Press 75/4(1996-04), 439-446 0032-5791 75:4<439 1996 75 ps https://doi.org/10.3382/ps.0750439 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.3382/ps.0750439 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- ZHU J. Department of Animal Sciences and aut NATIONALLICENCE 700 1- NESTOR K. E. Department of Animal Sciences and aut NATIONALLICENCE 700 1- PATTERSON R. A. Department of Animal Sciences and aut NATIONALLICENCE 700 1- JACKWOOD D. J. Food Animal Health Research Program, The Ohio State University, Ohio Agricultural Research and Development Center, Wooster, Ohio 44691 and aut NATIONALLICENCE 700 1- EMMERSON D. A. Hybrid Turkeys Inc., Kitchener, Ontario, Canada, N2B 3E9 aut NATIONALLICENCE 773 0- Poultry Science Oxford University Press 75/4(1996-04), 439-446 0032-5791 75:4<439 1996 75 ps Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford