caa a22 4500 397568649 CHVBK 20180308164835.0 cr unu---uuuuu 161202e199611 xx s 000 0 eng 10.3382/ps.0751365 doi (NATIONALLICENCE)oxford-10.3382/ps.0751365 Experimental Infection of Laying Hens with Salmonella enteritidis Strains that Express Different Types of Fimbriae [Elektronische Daten] [D. THIAGARAJAN, H. L. THACKER, A. M. SAEED] A study was conducted to compare the pathogenicity of three Salmonella enteritidis phage type 8 strains (9, 21, and 30) in 30-wk-old laying hens. Strain 9 expressed two types of fimbriae of 14 and 21 kDa. Strain 30 expressed a single fimbrial type (21 kDa). Strain 21 did not express any fimbrial protein. Laying hens were divided into three groups of 35 each and each group was orally inoculated with a single S. enteritidis strain (1 × 108 cfu per bird). Significantly less intensive cecal colonization and fecal shedding of the organism were observed in hens that were inoculated with the strain that did not express fimbriae than in birds inoculated with other two strains (P < 0.05). Isolation of S. enteritidis from liver, spleen, reproductive organs, and egg contents did not differ between groups. Mean serum S. enteritidis lipopolysaccharide-specific antibody titers of birds inoculated with strain 21 were lower than titers of hens that were inoculated with the other two strains from the 5th wk through the end of the trial. Immunoblot of the bacterial outer membrane structures revealed the presence of serum antibodies against lipopolysaccharide, membrane-associated proteins, and purified 14 kDa fimbrial protein in birds inoculated with strain 9 as late as 9 wk postinoculation. Results of this study are consistent with a role for fimbrial proteins in the cecal colonization by S. enteritidis. In addition, cecal colonization mediated by fimbrial proteins may enhance the elicitation of humoral immune response against S. enteritidis. © 1996 Poultry Science Association, Inc. Environment and Health nationallicence Salmonella enteritidis nationallicence hen nationallicence pathogenesis nationallicence ceca nationallicence colonization nationallicence THIAGARAJAN D. Department of Veterinary Pathobiology and aut THACKER H. L. Animal Disease Diagnostic Laboratory, School of Veterinary Medicine, Purdue University, West Lafayette, Indiana 47907 aut SAEED A. M. Department of Veterinary Pathobiology and aut Poultry Science Oxford University Press 75/11(1996-11), 1365-1372 0032-5791 75:11<1365 1996 75 ps https://doi.org/10.3382/ps.0751365 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.3382/ps.0751365 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- THIAGARAJAN D. Department of Veterinary Pathobiology and aut NATIONALLICENCE 700 1- THACKER H. L. Animal Disease Diagnostic Laboratory, School of Veterinary Medicine, Purdue University, West Lafayette, Indiana 47907 aut NATIONALLICENCE 700 1- SAEED A. M. Department of Veterinary Pathobiology and aut NATIONALLICENCE 773 0- Poultry Science Oxford University Press 75/11(1996-11), 1365-1372 0032-5791 75:11<1365 1996 75 ps Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford