caa a22 4500 397568681 CHVBK 20180308164835.0 cr unu---uuuuu 161202e199607 xx s 000 0 eng 10.3382/ps.0750943 doi (NATIONALLICENCE)oxford-10.3382/ps.0750943 Effect of Added Spermiophages in Pooled Turkey Semen on Fertility, Embryonic Mortality, and Hatchability [Elektronische Daten] [DEBRA A. BARNES, RONALD J. THURSTON, THOMAS R. SCOTT, NANCY KORN] The semen of turkeys with numerous spermiophages was used for isolating spermiophages by density gradient centrifugation. Isolated spermiophages were suspended in Beltsville Poultry Semen Extender (BPSE) and added to semen with low spermiophage numbers to give approximate spermiophage concentrations of: 2 × 105/mL (medium) and 106 (high). Semen with no added spermiophages was the control. Samples were diluted to 1:1 with BPSE, and for each spermiophage level (treatment), semen aliquots were either immediately inseminated or stored 6 h at 4 C with agitation (150 rpm) before insemination. Hens were inseminated weekly, and fertility, embryonic mortality, and hatchability of eggs were determined for a 10-wk period. The experiment was performed twice. In Trial 1, there were no differences in fertility between treatments except that fertility for control stored semen was lower (P ≤ 0.05) than that for fresh semen (89.27 vs 95.97, respectively; SEM = 2.2). Neither hatchability nor embryonic mortality was affected by spermiophage level in Trial 1. Spermiophages did not affect fertility in Trial 2; however, hatchability for unstored treatments with added spermiophages was significantly lower than for the control. For stored semen, hatchability was significantly (P ≤ 0.05) greater for treatments with added spermiophages than for the control. Differences in embryonic mortality in Trial 2 did not relate to adding spermiophages to the semen. No clearly defined detrimental effect of seminal spermiophages was shown in the present experiments. © 1996 Poultry Science Association, Inc. Physiology and Reproduction nationallicence turkey nationallicence spermiophage nationallicence fertility nationallicence reproduction nationallicence semen storage nationallicence BARNES DEBRA A. aut THURSTON RONALD J. 2To whom correspondence should be addressed. aut SCOTT THOMAS R. aut KORN NANCY aut Poultry Science Oxford University Press 75/7(1996-07), 943-948 0032-5791 75:7<943 1996 75 ps https://doi.org/10.3382/ps.0750943 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.3382/ps.0750943 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- BARNES DEBRA A. aut NATIONALLICENCE 700 1- THURSTON RONALD J. 2To whom correspondence should be addressed aut NATIONALLICENCE 700 1- SCOTT THOMAS R. aut NATIONALLICENCE 700 1- KORN NANCY aut NATIONALLICENCE 773 0- Poultry Science Oxford University Press 75/7(1996-07), 943-948 0032-5791 75:7<943 1996 75 ps Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford