caa a22 4500 397568975 CHVBK 20180308164836.0 cr unu---uuuuu 161202e199607 xx s 000 0 eng 10.3382/ps.0750900 doi (NATIONALLICENCE)oxford-10.3382/ps.0750900 Hepatic and Cardiac Oxidative Stress and Other Metabolic Changes in Broilers with the Ascites Syndrome [Elektronische Daten] [ANTONIO DÍAZ-CRUZ, CUAUHTÉMOC NAVA, RUBEN VILLANUEVA, MAURILIO SERRET, RAQUEL GUINZBERG, ENRIQUE PIÑA] The objective of this study was to evaluate the gluconeogenic response of in vitro stimulated hepatocytes from control broilers and broilers with clinical manifestations of the ascites syndrome. The basal rate of glucose synthesis from lactate was found to be threefold greater in sick birds than in the control group and stimulation obtained with epinephrine was found to be quantitatively similar in both groups. Under basal conditions, the hepatocytes from the sick broilers exhibited 60% more ammonium than the control birds. In addition, the quantification of thiobarbituric acid reactive substances, as indicators of cellular lipoperoxidation, showed an increase of over 100% in heart and liver of sick broilers fowl. In conclusion, the complex integrated response of gluconeogenesis to epinephrine is maintained in broilers with ascites, although their hepatocytes present changes compatible with those observed in cases of oxidative stress. It is not known whether this stress is a cause or a consequence of the ascites syndrome. © 1996 Poultry Science Association, Inc. Metabolism and Nutrition nationallicence ascites nationallicence broiler nationallicence free radicals nationallicence gluconeogenesis nationallicence oxidative stress nationallicence DÍAZ-CRUZ ANTONIO Department of Animal Nutrition and Biochemistry, School of Veterinary Medicine and Zootechnics, and aut NAVA CUAUHTÉMOC Department of Animal Nutrition and Biochemistry, School of Veterinary Medicine and Zootechnics, and aut VILLANUEVA RUBEN Department of Animal Nutrition and Biochemistry, School of Veterinary Medicine and Zootechnics, and aut SERRET MAURILIO Department of Animal Nutrition and Biochemistry, School of Veterinary Medicine and Zootechnics, and aut GUINZBERG RAQUEL Department of Biochemistry, School of Medicine, National Autonomous University of Mexico, Mexico 04510, D.F. Mexico aut PIÑA ENRIQUE Department of Biochemistry, School of Medicine, National Autonomous University of Mexico, Mexico 04510, D.F. Mexico aut Poultry Science Oxford University Press 75/7(1996-07), 900-903 0032-5791 75:7<900 1996 75 ps https://doi.org/10.3382/ps.0750900 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.3382/ps.0750900 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- DÍAZ-CRUZ ANTONIO Department of Animal Nutrition and Biochemistry, School of Veterinary Medicine and Zootechnics, and aut NATIONALLICENCE 700 1- NAVA CUAUHTÉMOC Department of Animal Nutrition and Biochemistry, School of Veterinary Medicine and Zootechnics, and aut NATIONALLICENCE 700 1- VILLANUEVA RUBEN Department of Animal Nutrition and Biochemistry, School of Veterinary Medicine and Zootechnics, and aut NATIONALLICENCE 700 1- SERRET MAURILIO Department of Animal Nutrition and Biochemistry, School of Veterinary Medicine and Zootechnics, and aut NATIONALLICENCE 700 1- GUINZBERG RAQUEL Department of Biochemistry, School of Medicine, National Autonomous University of Mexico, Mexico 04510, D.F. Mexico aut NATIONALLICENCE 700 1- PIÑA ENRIQUE Department of Biochemistry, School of Medicine, National Autonomous University of Mexico, Mexico 04510, D.F. Mexico aut NATIONALLICENCE 773 0- Poultry Science Oxford University Press 75/7(1996-07), 900-903 0032-5791 75:7<900 1996 75 ps Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford