caa a22 4500 397569017 CHVBK 20180308164836.0 cr unu---uuuuu 161202e199607 xx s 000 0 eng 10.3382/ps.0750910 doi (NATIONALLICENCE)oxford-10.3382/ps.0750910 Mesotocin Binding to Receptors in Hen Kidney Plasma Membranes [Elektronische Daten] [TETSUYA TAKAHASHI, MITSUO KAWASHIMA, A TADASHI YASUOKA, MICHIHARU KAMIYOSHI, KATUHIDE TANAKA] Radioligand assays were performed to demonstrate the presence of a receptor for mesotocin (MT) in the membrane fractions of the kidney of the hen. Specific [125I]MT bindings were decreased by the presence of Mg2+ and Ca2+, increased by the presence of EDTA, increased during the first 4 h of incubation and then reached a plateau, and increased with the increase in the protein concentration from 2.5 to 20 μg. The membrane fraction showed binding specificity to [125I]MT. The Scatchard plot revealed a curvilinear profile that indicated the presence of two classes of binding sites: a high affinity site and a low affinity site. The equilibrium dissociation constant was 0.08 ± 0.01 nM (χ̄ ± SEM; n = 5) in the high affinity site and 0.87 ± 0.08 nM (n = 5) in the low affinity site. The maximum binding capacity of the high and low affinity sites was 42 ± 4 and 129 ± 6 fmol/mg protein, respectively. The results suggest the presence of two distinct MT receptors in the kidney of the hen. © 1996 Poultry Science Association, Inc. Physiology and Reproduction nationallicence hen nationallicence kidney nationallicence mesotocin nationallicence mesotocin binding nationallicence mesotocin receptor nationallicence TAKAHASHI TETSUYA Department of Animal Science and Technology, Gifu University, Yanagido, Gifu 501-11, Japan aut KAWASHIMA MITSUO Department of Animal Science and Technology, Gifu University, Yanagido, Gifu 501-11, Japan aut YASUOKA A TADASHI Department of Oral and Maxillofacial Surgery, Gifu University School of Medicine, Tsukasamachi, Gifu 500, Japan aut KAMIYOSHI MICHIHARU Department of Animal Science and Technology, Gifu University, Yanagido, Gifu 501-11, Japan aut TANAKA KATUHIDE Department of Zootechnical Science, Tokyo University of Agriculture, Sakuragaoka, Setagaya-ku, Tokyo 156, Japan aut Poultry Science Oxford University Press 75/7(1996-07), 910-914 0032-5791 75:7<910 1996 75 ps https://doi.org/10.3382/ps.0750910 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.3382/ps.0750910 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- TAKAHASHI TETSUYA Department of Animal Science and Technology, Gifu University, Yanagido, Gifu 501-11, Japan aut NATIONALLICENCE 700 1- KAWASHIMA MITSUO Department of Animal Science and Technology, Gifu University, Yanagido, Gifu 501-11, Japan aut NATIONALLICENCE 700 1- YASUOKA A TADASHI Department of Oral and Maxillofacial Surgery, Gifu University School of Medicine, Tsukasamachi, Gifu 500, Japan aut NATIONALLICENCE 700 1- KAMIYOSHI MICHIHARU Department of Animal Science and Technology, Gifu University, Yanagido, Gifu 501-11, Japan aut NATIONALLICENCE 700 1- TANAKA KATUHIDE Department of Zootechnical Science, Tokyo University of Agriculture, Sakuragaoka, Setagaya-ku, Tokyo 156, Japan aut NATIONALLICENCE 773 0- Poultry Science Oxford University Press 75/7(1996-07), 910-914 0032-5791 75:7<910 1996 75 ps Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford