caa a22 4500 397569076 CHVBK 20180308164836.0 cr unu---uuuuu 161202e199603 xx s 000 0 eng 10.3382/ps.0750346 doi (NATIONALLICENCE)oxford-10.3382/ps.0750346 Antibody Response to Sheep Red Blood Cells in a Major Histocompatibility (B) Complex Aneuploid Line of Chickens [Elektronische Daten] [K. T. LEPAGE, S. E. BLOOM, R. L. TAYLOR] An integral part of the immune response is the production of antibodies specific for different antigenic challenges. Genes of the MHC encode products that regulate immunity. This study utilized the FCT-15 line of chickens, which is aneuploid for the chromosome containing the ribosomal RNA genes (rDNA) and the MHC or B complex to determine whether an antibody response to SRBC would vary as a function of B complex gene dose. Mating of trisomic parents (B 15 B 15 B 15) animals produced progeny having either a disomic (B 15 B 15), trisomic (B 15 B 15 B 15), or tetrasomic (B 15 B 15 B 15 B 15) B complex dosage. The number of B/rDNA chromosomes, and thus the B complex dosage, was determined by feather pulp nucleolar typing of chicks at hatch. A 5% SRBC antigenic challenge, which induces a T cell-dependent antibody response, was injected at 6 wk of age. Samples taken prior to SRBC injection as well as 5, 8, and 12 d postinjection were assayed for total and mercaptoethanol-resistant antibody. Peak antibody titers (log2), day of peak titer and rate of titer decline were calculated using a quadratic equation for each bird. Differences among the three B complex dosages were evaluated by analysis of variance. Antibody titers rose from 5 to 8 d postinjection and declined thereafter without significant differences among the three B complex doses. Calculations from the quadratic equations showed that B complex dose affected neither peak antibody titer nor day of peak titer. However, trisomic and tetrasomic animals had significantly more rapid rates of decline from the maximum titer. In aneuploid chickens, changes in antigen processing, antigen presentation, or persistence of processed antigen may maintain levels of antibody production found in disomic chickens and explain the more rapid decline of titer. © 1996 Poultry Science Association, Inc. Immunology nationallicence aneuploidy nationallicence B complex nationallicence antigen nationallicence immune response nationallicence major histocompatibility complex nationallicence LEPAGE K. T. Department of Animal and Nutritional Sciences, University of New Hampshire, Durham, New Hampshire 03824 and aut BLOOM S. E. Department of Avian and Aquatic Animal Medicine, Cornell University, Ithaca, New York 14853 aut TAYLOR R. L. Department of Animal and Nutritional Sciences, University of New Hampshire, Durham, New Hampshire 03824 and aut Poultry Science Oxford University Press 75/3(1996-03), 346-350 0032-5791 75:3<346 1996 75 ps https://doi.org/10.3382/ps.0750346 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.3382/ps.0750346 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- LEPAGE K. T. Department of Animal and Nutritional Sciences, University of New Hampshire, Durham, New Hampshire 03824 and aut NATIONALLICENCE 700 1- BLOOM S. E. Department of Avian and Aquatic Animal Medicine, Cornell University, Ithaca, New York 14853 aut NATIONALLICENCE 700 1- TAYLOR R. L. Department of Animal and Nutritional Sciences, University of New Hampshire, Durham, New Hampshire 03824 and aut NATIONALLICENCE 773 0- Poultry Science Oxford University Press 75/3(1996-03), 346-350 0032-5791 75:3<346 1996 75 ps Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford