caa a22 4500 397569106 CHVBK 20180308164836.0 cr unu---uuuuu 161202e199612 xx s 000 0 eng 10.3382/ps.0751536 doi (NATIONALLICENCE)oxford-10.3382/ps.0751536 Alkaline Phosphatase Activity in the Theca of Ovarian Follicles of the Hen Throughout Follicular Development [Elektronische Daten] [C. CHAPEAU, H. ENGELHARDT, G. J. KING, R. J. ETCHES] Within the hen's ovary, the theca tissue of small follicles that have not yet entered the preovulatory hierarchy is a major contributor to ovarian steroidogenesis. Relatively little is known about developmental stages in the theca layer within this pool of follicles and of the histological properties of this tissue. In pigs and sheep, alkaline phosphatase activity has been identified in the theca of preovulatory follicles and in theca-derived cells in the corpus luteum. The objectives of this study were to document morphological changes in the theca layer through pre- and postovulatory follicular development, and to assess alkaline phosphatase activity at these developmental stages. Ovarian tissue containing small white, large white, small yellow, large yellow, and postovulatory follicles (POF) was obtained from mature White Leghorn hens and embedded in either methacrylate for morphological assessment or paraffin for alkaline phosphatase histochemistry. Alkaline phosphatase activity was found to be specific for cells of the theca interna in preovulatory follicles. Activity was first detectable in very small white follicles, the earliest stage in which the theca interna could be distinguished from the theca externa, and highest in the yellow yolky follicles. After ovulation, activity decreased to undetectable levels by the POF4 to 5 stages in POF within 4 to 5 d after ovulation. This study has shown that in the domestic hen, alkaline phosphatase activity is present in cells adjacent to the basal lamina of the theca interna of preovulatory follicles and POF up to 4 to 5 d after ovulation. Identification of the functional significance of this histological reaction will provide new information on ovarian function in the hen. © 1996 Poultry Science Association, Inc. Physiology and Reproduction nationallicence alkaline phosphatase nationallicence ovary nationallicence theca nationallicence hen nationallicence follicle nationallicence CHAPEAU C. aut ENGELHARDT H. aut KING G. J. aut ETCHES R. J. aut Poultry Science Oxford University Press 75/12(1996-12), 1536-1545 0032-5791 75:12<1536 1996 75 ps https://doi.org/10.3382/ps.0751536 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.3382/ps.0751536 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- CHAPEAU C. aut NATIONALLICENCE 700 1- ENGELHARDT H. aut NATIONALLICENCE 700 1- KING G. J. aut NATIONALLICENCE 700 1- ETCHES R. J. aut NATIONALLICENCE 773 0- Poultry Science Oxford University Press 75/12(1996-12), 1536-1545 0032-5791 75:12<1536 1996 75 ps Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford