caa a22 4500 397569211 CHVBK 20180308164836.0 cr unu---uuuuu 161202e199612 xx s 000 0 eng 10.3382/ps.0751498 doi (NATIONALLICENCE)oxford-10.3382/ps.0751498 Dietary Lead Alters Fatty Acid Composition and Membrane Peroxidation in Chick Liver Microsomes [Elektronische Daten] [SCOTT O. KNOWLES, WILLIAM E. DONALDSON] Inorganic Pb acetate is a pro-oxidant, and peroxidation damage to cellular membrane lipids, leading to membrane fragility and permeability, is a likely consequence of Pb poisoning. In addition to the systemic peroxidation that occurs in vivo, Pb-contaminated feedstuffs can contribute preformed peroxides. Treatments with dietary Pb that have been shown to increase tissue peroxide levels in animals may be related to the consumption of preformed peroxides from the diet. In the current study, we evaluated the possible separate effects of feed and systemic peroxides by administering equivalent doses of Pb acetatetrihydrate to chicks via either 1,500 ppm Pb in the diet or via gastric intubation. Peroxidation of lipids in hepatic microsomal membranes (assessed as malonyldialdehyde production) from birds intoxicated with Pb by either route of administration was more than double that of untreated controls. Also, both routes of Pb exposure doubled the concentration of hepatic microsomal arachidonic acid, a peroxidizable polyunsaturated fatty acid. In the data reported here, we show that tissue peroxide levels are unaffected by the method of oral Pb administration and thus, by inference, independent of peroxide content of the feed. © 1996 Poultry Science Association, Inc. Environment and Health nationallicence lead nationallicence peroxidation nationallicence lipids nationallicence tissue peroxides nationallicence arachidonic acid nationallicence KNOWLES SCOTT O. aut DONALDSON WILLIAM E. 2 To whom correspondence should be addressed: Department of Poultry Science, Box 7608, North Carolina State University, Raleigh, NC 27695-7608. aut Poultry Science Oxford University Press 75/12(1996-12), 1498-1500 0032-5791 75:12<1498 1996 75 ps https://doi.org/10.3382/ps.0751498 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.3382/ps.0751498 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- KNOWLES SCOTT O. aut NATIONALLICENCE 700 1- DONALDSON WILLIAM E. 2 To whom correspondence should be addressed: Department of Poultry Science, Box 7608, North Carolina State University, Raleigh, NC 27695-7608 aut NATIONALLICENCE 773 0- Poultry Science Oxford University Press 75/12(1996-12), 1498-1500 0032-5791 75:12<1498 1996 75 ps Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford