caa a22 4500 397569440 CHVBK 20180308164837.0 cr unu---uuuuu 161202e199606 xx s 000 0 eng 10.3382/ps.0750691 doi (NATIONALLICENCE)oxford-10.3382/ps.0750691 Promoter and Transcription of Type X Collagen Gene in Broiler Chickens with Tibial Dyschondroplasia [Elektronische Daten] [X. ZHANG, G. R. MCDANIEL, J. J. GIAMBRONE, E. SMITH] Type × collagen is produced exclusively in hypertrophic chondrocytes of the growth plate of the proximal tibiotarsus and is believed to play an important role during normal development from chondrogenesis to osteogenesis. Chondrocytes of chickens with tibial dyschondroplasia (TD) fail to attain full hypertrophy and the amount of type × collagen, being a marker of hypertrophy, is likely to be reduced. It is not clear whether transcriptional regulation is functional for expression of the type × collagen gene in TD birds. Nucleotide sequence of the type × collagen gene promoter was determined by sequencing PCR-based DNA clones. Nucleotide identity of this fragment between the normal and TD carriers was 97.6%. Both normal and TD birds were similar in a putative transcription start site, the site of TATAA box, and neither had a CCAAT box. However, there were two gaps in TD carriers, four gaps in normals, and five nucleotide substitution sites. By rapid amplification of cDNA ends by PCR (RACE-PCR), transcription of the gene was assessed using total RNA and mRNA from both normal chondrocytes and TD lesions at 3 and 4 wk of age. The RACE-PCR product for type × collagen mRNA was detectable in both normal and TD birds at two stages. No difference was found between them. This result does not support the hypothesis that transcriptional regulation of type × collagen gene is important in TD development of chickens. Variations in the promoter region did not affect transcription of type × collagen gene in TD carrier chickens. © 1996 Poultry Science Association, Inc. Breeding and Genetics nationallicence chicken nationallicence promoter nationallicence tibial dyschondroplasia nationallicence type × collagen nationallicence ZHANG X. Department of Poultry Science, Alabama Agricultural Experiment Station, Auburn University, Alabama 36849-5416 aut MCDANIEL G. R. Department of Poultry Science, Alabama Agricultural Experiment Station, Auburn University, Alabama 36849-5416 aut GIAMBRONE J. J. Department of Poultry Science, Alabama Agricultural Experiment Station, Auburn University, Alabama 36849-5416 aut SMITH E. Department of Agriculture Sciences, Tuskegee University, Alabama 36083 aut Poultry Science Oxford University Press 75/6(1996-06), 691-694 0032-5791 75:6<691 1996 75 ps https://doi.org/10.3382/ps.0750691 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.3382/ps.0750691 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- ZHANG X. Department of Poultry Science, Alabama Agricultural Experiment Station, Auburn University, Alabama 36849-5416 aut NATIONALLICENCE 700 1- MCDANIEL G. R. Department of Poultry Science, Alabama Agricultural Experiment Station, Auburn University, Alabama 36849-5416 aut NATIONALLICENCE 700 1- GIAMBRONE J. J. Department of Poultry Science, Alabama Agricultural Experiment Station, Auburn University, Alabama 36849-5416 aut NATIONALLICENCE 700 1- SMITH E. Department of Agriculture Sciences, Tuskegee University, Alabama 36083 aut NATIONALLICENCE 773 0- Poultry Science Oxford University Press 75/6(1996-06), 691-694 0032-5791 75:6<691 1996 75 ps Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford