caa a22 4500 397569548 CHVBK 20180308164837.0 cr unu---uuuuu 161202e199612 xx s 000 0 eng 10.3382/ps.0751569 doi (NATIONALLICENCE)oxford-10.3382/ps.0751569 Developmental Stages of Primary Oocytes in Turkeys [Elektronische Daten] [J. L. CARLSON, M. R. BAKST, M. A. OTTINGER] Little is known about the growth and differentiation of the primary oocyte in the sexually mature chicken or turkey hen. In this study, primary oocytes from turkey hens in egg production were examined by light and electron microscopy. Based on oocyte and germinal vesicle (GV) diameters and organelle morphology and distribution, the sequential development of the primary oocyte was divided into five stages. No Balbiani body was observed in Stage I oocytes (< 80 μm in diameter). Pleomorphic mitochondria were localized around the GV and multivesicular bodies were scattered in the ooplasm. By Stage II (81 to 150 μm), the Balbiani body was observed adjacent to the GV. Pleomorphic mitochondria, macrobodies, and smooth endoplasmic reticulum (SER) were associated with the Balbiani body. Lipid droplets were predominantly localized to the periphery of the oocyte. The Balbiani body was partially dispersed by Stage III (151 to 350 μm) and associated organelles appeared in clusters in the ooplasm. Golgi and SER were observed immediately subjacent to the oolemma. Stage IV oocytes (351 to 500 μm) were characterized by the absence of the Balbiani body, a more centrally located GV, and the redistribution of the mitochondria to the periphery of the oocyte. Throughout the ooplasm was vesicular SER. By Stage V (501 to 800 μm), zonation of the organelles was completed with the mitochondrial ring immediately subjacent to the oolemma and a concentric layer of lipid droplets subjacent to the mitochondrial ring. The GV was in the periphery of the oocyte. Organelle and inclusion redistribution and organelle pleomorphism were presumed to be reflective of increasing metabolic and transport requirements of the growing oocyte in the mature turkey hen. © 1996 Poultry Science Association, Inc. Physiology and Reproduction nationallicence oocyte maturation nationallicence germinal vesicle nationallicence meiosis nationallicence oogenesis nationallicence turkey hen nationallicence CARLSON J. L. Department of Poultry Science, University of Maryland, College Park, Maryland 20742 and aut BAKST M. R. Germplasm and Gamete Physiology Laboratory, Agricultural Research Service, USDA, Beltsville, Maryland 20705-2350 aut OTTINGER M. A. Department of Poultry Science, University of Maryland, College Park, Maryland 20742 and aut Poultry Science Oxford University Press 75/12(1996-12), 1569-1578 0032-5791 75:12<1569 1996 75 ps https://doi.org/10.3382/ps.0751569 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.3382/ps.0751569 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- CARLSON J. L. Department of Poultry Science, University of Maryland, College Park, Maryland 20742 and aut NATIONALLICENCE 700 1- BAKST M. R. Germplasm and Gamete Physiology Laboratory, Agricultural Research Service, USDA, Beltsville, Maryland 20705-2350 aut NATIONALLICENCE 700 1- OTTINGER M. A. Department of Poultry Science, University of Maryland, College Park, Maryland 20742 and aut NATIONALLICENCE 773 0- Poultry Science Oxford University Press 75/12(1996-12), 1569-1578 0032-5791 75:12<1569 1996 75 ps Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford