caa a22 4500 397569610 CHVBK 20180308164837.0 cr unu---uuuuu 161202e199610 xx s 000 0 eng 10.1093/oxfordjournals.jmicro.a023459 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jmicro.a023459 Phenotypic Characteristics and Significance of Reticular Meshwork Surrounding Splenic White Pulp of Mice [Elektronische Daten] [Hisako Tanaka, Yoshiaki Hataba, Saburo Saito, Osamu Fukushima, Masayuki Miyasaka] This report describes the phenotypic characteristics of the reticular meshwork (RM) localized between the marginal zone and white pulp of mice. Considered from an anatomical point of view, RM contributes to the migration of lymphocytes into splenic white pulp. We divided the RM into two parts: one surrounding the periarterial lymphoid sheaths (PALS) and the other surrounding the follicles. Throughout both locations of RM, alkaline phosphatase activity was noted on the plasma membrane of the reticular cells and tenascin was expressed on the collagen fibrils in the matrix of the reticular fibers. In contrast, the expression of MAdCAM-1, one of the homing receptors of lymphocytes, was solely confined to the plasma membranes of the fiber-forming reticular cells and processes of the perifollicular RM. In aly mice with defective perifollicular RM and loss of MAdCAM-1 expression, lymphocyte homing to the white pulp was remarkably inhibited, and the compartmentalization of T and B cells did not occur. This fact supports the notion that perifollicular RM plays an essential role in lymphocyte homing and compartmentalization. © 1996 Oxford University Press Full-length Papers nationallicence splenic white pulp nationallicence reticular meshwork nationallicence alkaline phosphatase nationallicence MAdCAM-1 nationallicence tenascin nationallicence Tanaka Hisako Division of Molecular Cell Biology, The Jikel University School of Medicine, 3-28-5 Nishi-shinbashi, Minato-ku, Tokyo, 105 Japan aut Hataba Yoshiaki Division of Molecular Cell Biology, The Jikel University School of Medicine, 3-28-5 Nishi-shinbashi, Minato-ku, Tokyo, 105 Japan aut Saito Saburo Division of Molecular Immunology, Institute of DNA Medicine, The Jikel University School of Medicine 3-28-5 Nishi-shinbashi, Minato-ku, Tokyo, 105 Japan aut Fukushima Osamu First Department of Anatomy, The Jikel University School of Medicine 3-28-5 Nishi-shinbashi, Minato-ku, Tokyo, 105 Japan aut Miyasaka Masayuki Department of Bioregulation, Osaka University School of Medicine Suita, Osaka 565, Japan aut Microscopy Oxford University Press 45/5(1996-10), 407-416 0022-0744 45:5<407 1996 45 jmicro https://doi.org/10.1093/oxfordjournals.jmicro.a023459 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jmicro.a023459 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tanaka Hisako Division of Molecular Cell Biology, The Jikel University School of Medicine, 3-28-5 Nishi-shinbashi, Minato-ku, Tokyo, 105 Japan aut NATIONALLICENCE 700 1- Hataba Yoshiaki Division of Molecular Cell Biology, The Jikel University School of Medicine, 3-28-5 Nishi-shinbashi, Minato-ku, Tokyo, 105 Japan aut NATIONALLICENCE 700 1- Saito Saburo Division of Molecular Immunology, Institute of DNA Medicine, The Jikel University School of Medicine 3-28-5 Nishi-shinbashi, Minato-ku, Tokyo, 105 Japan aut NATIONALLICENCE 700 1- Fukushima Osamu First Department of Anatomy, The Jikel University School of Medicine 3-28-5 Nishi-shinbashi, Minato-ku, Tokyo, 105 Japan aut NATIONALLICENCE 700 1- Miyasaka Masayuki Department of Bioregulation, Osaka University School of Medicine Suita, Osaka 565, Japan aut NATIONALLICENCE 773 0- Microscopy Oxford University Press 45/5(1996-10), 407-416 0022-0744 45:5<407 1996 45 jmicro Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford