caa a22 4500 397569726 CHVBK 20180308164837.0 cr unu---uuuuu 161202e199610 xx s 000 0 eng 10.1093/oxfordjournals.jmicro.a023456 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jmicro.a023456 Ultrastructure of Resting and rh-GMCSF-Treated Human Macrophages Derived from Blood Monocytes [Elektronische Daten] [Sameh El Shewemi, Fahad Al-Shammary, Faten Al-Zamel, Rafique Soliman] The ultrastructure of cultured blood mondcyte-derived human macrophages was investigated and correlated under the effect of different doses of rh-GMCSF (dose 1=25IU/ml, dose 2 = 125IU/ml and dose 3 = 250IU/ml). Resting macrophages showed irregular cell borders and pseudopodia pushed out in all directions. Their cytoplasm depicted rough endoplasmic reticulum and Golgi complex in the perinuclear area. Lipid globules, primary lysosomes and mitochondria were characteristically prominent. rh-GMCSF-stimulated macrophages were more voluminous and their nuclei were irregular in outline, with predominance of euochromatin over hetero-chromatin. The cytoplasm was overcrowded by an increasing number of organelles including lysosomes, phagolysosomes and mitochondria. Golgi complex demonstrated a wide-spread distribution along the cells, with profound membrane expansion and cisternal dilatation; especially, in cells treated with dose 2. Electron dense osmiophilic deposits (collapsed membranes) were seen in association with lipid globules, which were commonly polarized at cell peripheries. Most of these changes were dose dependent. However, cells treated with dose 3 manifested additionally well-developed centrioles, inapparent nuclear membrane, display of microfilaments and well-established adhesions. The demonstrated ultrastructural changes in rh-GMCSF-treated human macrophages indicated pronounced activation, which supports the reported clinical effect of this cytokine. © 1996 Oxford University Press Full-length Papers nationallicence macrophage nationallicence ultrastructure nationallicence rh-GMCSF nationallicence Shewemi Sameh El Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 11433, Kingdom of Saudi Arabia aut Al-Shammary Fahad Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 11433, Kingdom of Saudi Arabia aut Al-Zamel Faten Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 11433, Kingdom of Saudi Arabia aut Soliman Rafique Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 11433, Kingdom of Saudi Arabia aut Microscopy Oxford University Press 45/5(1996-10), 388-394 0022-0744 45:5<388 1996 45 jmicro https://doi.org/10.1093/oxfordjournals.jmicro.a023456 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jmicro.a023456 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shewemi Sameh El Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 11433, Kingdom of Saudi Arabia aut NATIONALLICENCE 700 1- Al-Shammary Fahad Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 11433, Kingdom of Saudi Arabia aut NATIONALLICENCE 700 1- Al-Zamel Faten Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 11433, Kingdom of Saudi Arabia aut NATIONALLICENCE 700 1- Soliman Rafique Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 11433, Kingdom of Saudi Arabia aut NATIONALLICENCE 773 0- Microscopy Oxford University Press 45/5(1996-10), 388-394 0022-0744 45:5<388 1996 45 jmicro Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford