caa a22 4500 397569793 CHVBK 20180308164838.0 cr unu---uuuuu 161202e199608 xx s 000 0 eng 10.1093/oxfordjournals.jmicro.a023442 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jmicro.a023442 Nagata Tetsuji Department of Anatomy and Cell Biology, Shinshu University School of Medicine, Matsumoto, 390 Japan Techniques and Application of Electron Microscopic Radioautography [Elektronische Daten] [Tetsuji Nagata] The techniques for electron microscopic radioautography can be divided into two categories, i.e., chemical fixation followed by wet-mounting radioautography and cryo-fixation followed by dry-mounting radioautography. The former procedure is limited in application to only insoluble compounds, while the latter is universally applicable to any kind of compound including soluble compounds. The both procedures, the chemical fixation wet-mounting procedure and the cryo-fixation dry-mounting procedure at the electron microscopic level, which were developed in our laboratory, are described in detail in this article. The applications of both the procedures are also briefly mentioned. Chemical fixation with glutaraldehyde and osmium tetroxide followed by wet-mounting radioautography demonstrates insoluble macromolecular synthesis such as nucleic acids (both DNA and RNA), proteins, glucides and lipids. On the other hand, cryo-fixation followed by dry-mounting radioautography demonstrates soluble small molecular compounds such as macromolecular precursors, or hormones, vitamins, drugs and inorganic compounds. These procedures are expected to be applied for various organic and inorganic substances in living organisms to clarify the sites of their incorporation, synthesis and discharge. © 1996 Oxford University Press Review nationallicence radioautography nationallicence DNA nationallicence RNA nationallicence protein nationallicence synthesis nationallicence Microscopy Oxford University Press 45/4(1996-08), 258-274 0022-0744 45:4<258 1996 45 jmicro https://doi.org/10.1093/oxfordjournals.jmicro.a023442 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jmicro.a023442 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Nagata Tetsuji Department of Anatomy and Cell Biology, Shinshu University School of Medicine, Matsumoto, 390 Japan NATIONALLICENCE 773 0- Microscopy Oxford University Press 45/4(1996-08), 258-274 0022-0744 45:4<258 1996 45 jmicro Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford