caa a22 4500 39756984X CHVBK 20180308164838.0 cr unu---uuuuu 161202e199608 xx s 000 0 eng 10.1093/oxfordjournals.jmicro.a023450 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jmicro.a023450 Translocation of Hepatitis B Virus Core Particles through Nuclear Pores in Transformed Yeast Cells [Elektronische Daten] [Masashi Yamaguchi, Yoshinobu Miyatsu, Hiroshi Mizokami, Lele Matsuoka, Kanji Takeo] In our previous transmission electron microscopic study of hepatitis B virus core antigen in transformed yeast cells, we observed core particles passing through the nuclear pores. We have now analyzed 1,421 nuclear pores in transformed yeast, and conclude that 1) translocation of core particles from the nucleus to the cytoplasm occurs through the nuclear pores; 2) translocation sites are located in the center of nuclear pores; 3) at least 95% of pores are involved in the translocation process; 4) proteins as large as 28 nm in diameter can cross the envelope; 5) translocation does not stop, but rather becomes more active during nuclear division in yeast cells. © 1996 Oxford University Press Note nationallicence hepatitis B virus core particles nationallicence nuclear pore nationallicence translocation nationallicence yeast cells nationallicence Yamaguchi Masashi Department of Research and Development. The Chemo-Sero-Therapeutic Research Institute, Shimizu, Kumamoto, 860 Japan, 3-25-8 Nishi-Shinbashi, Minato-ku, Tokyo, 105 Japan, 1-8-1 Inohana, Chuo-ku, Chiba, 260 Japan aut Miyatsu Yoshinobu Department of Research and Development. The Chemo-Sero-Therapeutic Research Institute Shimizu, Kumamoto, 860 Japan aut Mizokami Hiroshi Department of Research and Development. The Chemo-Sero-Therapeutic Research Institute Shimizu, Kumamoto, 860 Japan aut Matsuoka Lele Molecular Cell Biology, Institute of DMA Medicine. The Jikei University School of Medicine 3-25-8 Nishi-Shinbashi, Minato-ku, Tokyo, 105 Japan aut Takeo Kanji Department of Research and Development. The Chemo-Sero-Therapeutic Research Institute, Shimizu, Kumamoto, 860 Japan, 3-25-8 Nishi-Shinbashi, Minato-ku, Tokyo, 105 Japan, 1-8-1 Inohana, Chuo-ku, Chiba, 260 Japan aut Microscopy Oxford University Press 45/4(1996-08), 321-324 0022-0744 45:4<321 1996 45 jmicro https://doi.org/10.1093/oxfordjournals.jmicro.a023450 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jmicro.a023450 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yamaguchi Masashi Department of Research and Development. The Chemo-Sero-Therapeutic Research Institute, Shimizu, Kumamoto, 860 Japan, 3-25-8 Nishi-Shinbashi, Minato-ku, Tokyo, 105 Japan, 1-8-1 Inohana, Chuo-ku, Chiba, 260 Japan aut NATIONALLICENCE 700 1- Miyatsu Yoshinobu Department of Research and Development. The Chemo-Sero-Therapeutic Research Institute Shimizu, Kumamoto, 860 Japan aut NATIONALLICENCE 700 1- Mizokami Hiroshi Department of Research and Development. The Chemo-Sero-Therapeutic Research Institute Shimizu, Kumamoto, 860 Japan aut NATIONALLICENCE 700 1- Matsuoka Lele Molecular Cell Biology, Institute of DMA Medicine. The Jikei University School of Medicine 3-25-8 Nishi-Shinbashi, Minato-ku, Tokyo, 105 Japan aut NATIONALLICENCE 700 1- Takeo Kanji Department of Research and Development. The Chemo-Sero-Therapeutic Research Institute, Shimizu, Kumamoto, 860 Japan, 3-25-8 Nishi-Shinbashi, Minato-ku, Tokyo, 105 Japan, 1-8-1 Inohana, Chuo-ku, Chiba, 260 Japan aut NATIONALLICENCE 773 0- Microscopy Oxford University Press 45/4(1996-08), 321-324 0022-0744 45:4<321 1996 45 jmicro Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford