caa a22 4500 397570171 CHVBK 20180308164839.0 cr unu---uuuuu 161202e199612 xx s 000 0 eng 10.1093/oxfordjournals.jmicro.a023470 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jmicro.a023470 Distinct Distribution of Actin Microfilaments in Agar Colony-Forming Cells Harboring Different Metastatic Ability [Elektronische Daten] [Yoichi Jinguji, Shinobu Nakamura, Takahiro Nomura] The distribution of actin microfilaments in two cell lines, ras/mycSFME and r/mHM-SFME-1, was studied under colony-forming conditions in soft agar gel. Both cell lines were transformed by human c-Ha-ras and mouse c-myc genes. The r/mHM-SFME-1 cell line, which is derived from ras/mycSFME, shows highly metastatic potential in host animals, whereas ras/mycSFME shows low potential. Colonies of the r/mHM-SFME-1 cell consisted of loosely packed cells with dispersed satellite cells around the colony, whereas the ras/mycSFME cells produced fairly compacted colonies. The distribution of actin microfilaments in the submembranous region, especially at the cell-cell adhesion site, was more frequent in the ras/mycSFME cells than that of the highly metastatic r/mHM-SFME-1 cells. The cell-cell adherens junction developed well in the ras/mycSFME cells as compared to that of the r/mHM-SFME-1 cells. When these cell lines were cultured on fibronectin-coated dishes, however, both expressed a fibroblastic appearance and spread well over the dishes. Therefore, the phenotypic differences in the metastatic potentials of the two cell lines were demonstrated by the three-dimensional cultures in soft agar gel. © 1996 Oxford University Press Full-length Papers nationallicence actin microfilament nationallicence adherens junction nationallicence agar colony nationallicence metastatic ability nationallicence Jinguji Yoichi Gunma Prefectural College of Health Sciences 323-1 Kamioki, Maebashi, Gunma, 371 Japan aut Nakamura Shinobu 3rd Department of Internal Medicine, School of Medicine, Cancer Research Institute, Kanazawa University 13-1 Takara-machi, Kanazawa, Ishikawa, 920 Japan aut Nomura Takahiro Department of Molecular Biology, Cancer Research Institute, Kanazawa University 13-1 Takara-machi, Kanazawa, Ishikawa, 920 Japan aut Microscopy Oxford University Press 45/6(1996-12), 471-476 0022-0744 45:6<471 1996 45 jmicro https://doi.org/10.1093/oxfordjournals.jmicro.a023470 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jmicro.a023470 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jinguji Yoichi Gunma Prefectural College of Health Sciences 323-1 Kamioki, Maebashi, Gunma, 371 Japan aut NATIONALLICENCE 700 1- Nakamura Shinobu 3rd Department of Internal Medicine, School of Medicine, Cancer Research Institute, Kanazawa University 13-1 Takara-machi, Kanazawa, Ishikawa, 920 Japan aut NATIONALLICENCE 700 1- Nomura Takahiro Department of Molecular Biology, Cancer Research Institute, Kanazawa University 13-1 Takara-machi, Kanazawa, Ishikawa, 920 Japan aut NATIONALLICENCE 773 0- Microscopy Oxford University Press 45/6(1996-12), 471-476 0022-0744 45:6<471 1996 45 jmicro Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford