caa a22 4500 397570228 CHVBK 20180308164839.0 cr unu---uuuuu 161202e199604 xx s 000 0 eng 10.1093/oxfordjournals.jmicro.a023420 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jmicro.a023420 Recent Advances in Molecular Histochemical Techniques: In Situ Hybridization and Southwestern Histochemistry [Elektronische Daten] [Takehiko Koji, Paul K. Nakane] Over the past decade, considerable efforts to understand the states of specific gene expression at cellular and/or subcellular levels have been made. For this particular purpose, nonradioactive in situ hybridization to localize mRNAs has been developed and improved substantially, and it is now recognized as a powerful, established light-microscopical technique. In this review, we focus on the recent advances in the technological aspects of nonradioactive in situ hybridization including the use of synthetic oligodeoxynucleotide probes, the progress in analysis of signals, and the application to electron microscopy. Also, southwestern histochemistry, a relatively new method of localizing transcription regulatory proteins by utilizing haptenized DNA with responsive element sequences is described. Then we discuss what we can see by combining these molecular histochemical methods which were brought about by the merger of molecular biology and structural biology. © 1996 Oxford University Press Review nationallicence in situ hybridization nationallicence T-T dimer nationallicence oligo-DNA nationallicence ultrastractural localization nationallicence southwestern histochemistry nationallicence Koji Takehiko Department of Anatomy (III), Nagasaki University School of Medicine, Sakamoto, Nagasaki, 852 Japan aut Nakane Paul K. Department of Anatomy (III), Nagasaki University School of Medicine, Sakamoto, Nagasaki, 852 Japan aut Microscopy Oxford University Press 45/2(1996-04), 119-127 0022-0744 45:2<119 1996 45 jmicro https://doi.org/10.1093/oxfordjournals.jmicro.a023420 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jmicro.a023420 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Koji Takehiko Department of Anatomy (III), Nagasaki University School of Medicine, Sakamoto, Nagasaki, 852 Japan aut NATIONALLICENCE 700 1- Nakane Paul K. Department of Anatomy (III), Nagasaki University School of Medicine, Sakamoto, Nagasaki, 852 Japan aut NATIONALLICENCE 773 0- Microscopy Oxford University Press 45/2(1996-04), 119-127 0022-0744 45:2<119 1996 45 jmicro Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford