caa a22 4500 397570244 CHVBK 20180308164839.0 cr unu---uuuuu 161202e199604 xx s 000 0 eng 10.1093/oxfordjournals.jmicro.a023427 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jmicro.a023427 Kondo Shunzo Mitsubishi Kasei Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo, 194 Japan Microinfusion Method Using Barium Sulfate for Visualization of Embryonic Blood Vessels with Light and Scanning Electron Microscopy [Elektronische Daten] [Shunzo Kondo] An X-ray contrast medium commonly used for clinical examination of the gastrointestinal tract was purified and applied as an injectant for the observation of the blood vasculature maturing in an early embryonic stage of the mouse. The embryos were perfused with the injectant through the umbilical cord and were processed for light and scanning electron microscope (LM and SEM) observations. When infused, the vessels turned white, the color of barium sulfate, thereby the adequate injection pressure and the amount of the injectant were readily controlled. The whole vasculature of the embryo was elucidated with an SEM in great detail at 9.5 days of the embryonic stage, four days earlier than previously achieved with a resin injection. The back scattered electron image of the lung alveolar capillaries containing barium sulfate was successfully obtained with an SEM in the 17.5 day embryo. The backscattering image and the secondary electron image of the identical area were compared to elucidate the spatial relationship between the capillaries and their circumferential cells such as type I and type II alveolar cells. The vascular injection with barium sulfate introduced may facilitate the systematic study of the blood vasculature and may provide useful information as to the relationship between vascular formation and organogenesis occurring in an early embryonic stage. © 1996 Oxford University Press Notes nationallicence barium sulfate nationallicence blood vessels nationallicence embryo nationallicence light microscope nationallicence SEM nationallicence Microscopy Oxford University Press 45/2(1996-04), 163-167 0022-0744 45:2<163 1996 45 jmicro https://doi.org/10.1093/oxfordjournals.jmicro.a023427 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jmicro.a023427 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Kondo Shunzo Mitsubishi Kasei Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo, 194 Japan NATIONALLICENCE 773 0- Microscopy Oxford University Press 45/2(1996-04), 163-167 0022-0744 45:2<163 1996 45 jmicro Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford