caa a22 4500 397570287 CHVBK 20180308164839.0 cr unu---uuuuu 161202e199604 xx s 000 0 eng 10.1093/oxfordjournals.jmicro.a023429 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jmicro.a023429 Preparation of Liposomes That Mimic the Membrane of Endoplasmic Reticulum of Rat Hepatocytes [Elektronische Daten] [Jun Watanabe, Youko Asaka, Kazuto Mino, Shinsuke Kanamura] To examine the interaction between biomembranes and membrane-bound proteins, large unilamellar liposomes have been required. In the present study, we prepared liposomes from a mixture of phospholipids having a phospholipid composition similar to that in the endoplasmic reticula (microsomes) of rat hepatocytes by eight different methods. The resulting liposomes were examined by a combination of the freeze-fracture-replica procedure with biochemical methods. The freeze-thawing method of Pick (1981) gave the best results; large unilamellar liposomes that mimic the membrane of endoplasmic reticulum were obtained. Liposomes made by this method are thus suitable for analysis of the interaction between the endoplasmic reticulum membrane and membrane-bound proteins. © 1996 Oxford University Press Notes nationallicence liposome nationallicence endoplasmic reticulum nationallicence microsome nationallicence freeze-fracture nationallicence hepatocytes nationallicence Watanabe Jun Department of Anatomy, Kansai Medical University, Moriguchi, Osaka, 570 Japan aut Asaka Youko Department of Anatomy, Kansai Medical University, Moriguchi, Osaka, 570 Japan aut Mino Kazuto Department of Anatomy, Kansai Medical University, Moriguchi, Osaka, 570 Japan aut Kanamura Shinsuke Department of Anatomy, Kansai Medical University, Moriguchi, Osaka, 570 Japan aut Microscopy Oxford University Press 45/2(1996-04), 171-176 0022-0744 45:2<171 1996 45 jmicro https://doi.org/10.1093/oxfordjournals.jmicro.a023429 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jmicro.a023429 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Watanabe Jun Department of Anatomy, Kansai Medical University, Moriguchi, Osaka, 570 Japan aut NATIONALLICENCE 700 1- Asaka Youko Department of Anatomy, Kansai Medical University, Moriguchi, Osaka, 570 Japan aut NATIONALLICENCE 700 1- Mino Kazuto Department of Anatomy, Kansai Medical University, Moriguchi, Osaka, 570 Japan aut NATIONALLICENCE 700 1- Kanamura Shinsuke Department of Anatomy, Kansai Medical University, Moriguchi, Osaka, 570 Japan aut NATIONALLICENCE 773 0- Microscopy Oxford University Press 45/2(1996-04), 171-176 0022-0744 45:2<171 1996 45 jmicro Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford