caa a22 4500 397570309 CHVBK 20180308164839.0 cr unu---uuuuu 161202e199606 xx s 000 0 eng 10.1093/oxfordjournals.jmicro.a023431 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jmicro.a023431 Developmental Profiles of Synaptophysin in Granule Cells of Rat Cerebellum: An Immunohistocytochemical Study [Elektronische Daten] [Mitsuaki Fujita, Tomoko Kadota, Toshio Sato] The expression of synaptophysin was immunohistocytochemically examined at the protein level in the cerebellar cortex of the neonatal rat with special reference to the development of granule cells. In the premigratory zone of the external granular layer, these cells expressed synaptophysin at an early stage of development, where it was localized in the trans-side cisterns of the Golgi apparatus and in nearby small vesicles. When the granule cells began to horizontally extend their axons in the upper molecular layer, the growth cones and expansions of parallel fibers already contained many small vesicles similar in shape and size to synaptic vesicles. These small vesicles were labeled with antibody against synaptophysin. Tubular structures were often found among the accumulations of synaptic vesicles, and their membranes were also labeled with synaptophysin. Both growth cones and expansions of parallel fibers were often observed to approach and to contact with the small processes of Purkinje cell dendrites. The contact between the two components formed a junction with the characteristics of a synapse. The profiles of synaptogenesis began to be observed in the deeper molecular layer during a period from the 7th to the 9th days after birth. © 1996 Oxford University Press Full-length Papers nationallicence synaptophysin nationallicence synaptic vesicles nationallicence granule cells nationallicence cerebellum nationallicence immunohistocytochemistry nationallicence Fujita Mitsuaki Department of Anatomy, Chiba University School of Medicine, Inohana 1-8-1, Chuo-ku, Chiba, 260 Japan, Inohana 1-8-1, Chuo-ku, Chiba, 260 Japan aut Kadota Tomoko Department of Anatomy, Chiba University School of Medicine Inohana 1-8-1, Chuo-ku, Chiba, 260 Japan aut Sato Toshio Department of Anatomy, Chiba University School of Medicine, Inohana 1-8-1, Chuo-ku, Chiba, 260 Japan, Inohana 1-8-1, Chuo-ku, Chiba, 260 Japan aut Microscopy Oxford University Press 45/3(1996-06), 185-194 0022-0744 45:3<185 1996 45 jmicro https://doi.org/10.1093/oxfordjournals.jmicro.a023431 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jmicro.a023431 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fujita Mitsuaki Department of Anatomy, Chiba University School of Medicine, Inohana 1-8-1, Chuo-ku, Chiba, 260 Japan, Inohana 1-8-1, Chuo-ku, Chiba, 260 Japan aut NATIONALLICENCE 700 1- Kadota Tomoko Department of Anatomy, Chiba University School of Medicine Inohana 1-8-1, Chuo-ku, Chiba, 260 Japan aut NATIONALLICENCE 700 1- Sato Toshio Department of Anatomy, Chiba University School of Medicine, Inohana 1-8-1, Chuo-ku, Chiba, 260 Japan, Inohana 1-8-1, Chuo-ku, Chiba, 260 Japan aut NATIONALLICENCE 773 0- Microscopy Oxford University Press 45/3(1996-06), 185-194 0022-0744 45:3<185 1996 45 jmicro Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford