caa a22 4500 397570325 CHVBK 20180308164839.0 cr unu---uuuuu 161202e199606 xx s 000 0 eng 10.1093/oxfordjournals.jmicro.a023436 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jmicro.a023436 Electron Microscopic Observations of Aberrant Capsids of Pseudorabies Virus [Elektronische Daten] [Yuko Muraki, Masao Yamada, Mariko Yoshida, Shunji Yamada, Jorge Padilla, Yoshihiro Hatano, Yoshio Hiramatsu, Fumio Uno, Shiro Nii] Interactions between four strains of pseudorabies virus (PrV) and seven kinds of cell lines were examined. Three kinds of cells (SKL, CPK and PK-15) were especially infected with PrV at an MOI of about 10 PFU/cell. At sequential intervals after infection, cells of these types were collected for electron microscopic observations and the infectious doses of culture fluids were assayed. Developmental features of PrV were found to be very similar to those of herpes simplex virus (HSV), with the slight difference that PrV developed a little earlier and more vigorously than did HSV. At the late stage of infection, aberrant capsids of PrV were observed frequently in the nucleus of SKL but rarely in the nuclei of CPK and PK-15. The titer of infectious virus produced by SKL was much larger than that produced by CPK and PK-15. Immuno-electron microscopic examination using a monoclonal antibody against the major capsid protein of PrV clearly demonstrated that the complete and aberrant capsids have a common epitope. The mechanism of the formation of aberrant capsids is discussed. © 1996 Oxford University Press Full-length Papers nationallicence aberrant capsid nationallicence herpesvirus nationallicence immuno-electron microscopy nationallicence pseudorabies virus nationallicence virus morphology nationallicence Muraki Yuko National Institute of Animal Health, Tsukuba Science City, Ibaraki, 305 Japan, 1263-2 Nishigata, Niimi, Okayama, 718 Japan, 2-5-1 Shikatacho, Okayama, 700 Japan aut Yamada Masao National Institute of Animal Health, Tsukuba Science City, Ibaraki, 305 Japan, 1263-2 Nishigata, Niimi, Okayama, 718 Japan, 2-5-1 Shikatacho, Okayama, 700 Japan aut Yoshida Mariko National Institute of Animal Health, Tsukuba Science City, Ibaraki, 305 Japan, 1263-2 Nishigata, Niimi, Okayama, 718 Japan, 2-5-1 Shikatacho, Okayama, 700 Japan aut Yamada Shunji National Institute of Animal Health, Tsukuba Science City, Ibaraki 305 Japan aut Padilla Jorge National Institute of Animal Health, Tsukuba Science City, Ibaraki, 305 Japan, 1263-2 Nishigata, Niimi, Okayama, 718 Japan, 2-5-1 Shikatacho, Okayama, 700 Japan aut Hatano Yoshihiro National Institute of Animal Health, Tsukuba Science City, Ibaraki, 305 Japan, 1263-2 Nishigata, Niimi, Okayama, 718 Japan, 2-5-1 Shikatacho, Okayama, 700 Japan aut Hiramatsu Yoshio National Institute of Animal Health, Tsukuba Science City, Ibaraki, 305 Japan, 1263-2 Nishigata, Niimi, Okayama, 718 Japan, 2-5-1 Shikatacho, Okayama, 700 Japan aut Uno Fumio Niimi Women's College 1263-2 Nishigata, Niimi, Okayama, 718 Japan aut Nii Shiro National Institute of Animal Health, Tsukuba Science City, Ibaraki, 305 Japan, 1263-2 Nishigata, Niimi, Okayama, 718 Japan, 2-5-1 Shikatacho, Okayama, 700 Japan aut Microscopy Oxford University Press 45/3(1996-06), 223-231 0022-0744 45:3<223 1996 45 jmicro https://doi.org/10.1093/oxfordjournals.jmicro.a023436 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jmicro.a023436 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Muraki Yuko National Institute of Animal Health, Tsukuba Science City, Ibaraki, 305 Japan, 1263-2 Nishigata, Niimi, Okayama, 718 Japan, 2-5-1 Shikatacho, Okayama, 700 Japan aut NATIONALLICENCE 700 1- Yamada Masao National Institute of Animal Health, Tsukuba Science City, Ibaraki, 305 Japan, 1263-2 Nishigata, Niimi, Okayama, 718 Japan, 2-5-1 Shikatacho, Okayama, 700 Japan aut NATIONALLICENCE 700 1- Yoshida Mariko National Institute of Animal Health, Tsukuba Science City, Ibaraki, 305 Japan, 1263-2 Nishigata, Niimi, Okayama, 718 Japan, 2-5-1 Shikatacho, Okayama, 700 Japan aut NATIONALLICENCE 700 1- Yamada Shunji National Institute of Animal Health, Tsukuba Science City, Ibaraki 305 Japan aut NATIONALLICENCE 700 1- Padilla Jorge National Institute of Animal Health, Tsukuba Science City, Ibaraki, 305 Japan, 1263-2 Nishigata, Niimi, Okayama, 718 Japan, 2-5-1 Shikatacho, Okayama, 700 Japan aut NATIONALLICENCE 700 1- Hatano Yoshihiro National Institute of Animal Health, Tsukuba Science City, Ibaraki, 305 Japan, 1263-2 Nishigata, Niimi, Okayama, 718 Japan, 2-5-1 Shikatacho, Okayama, 700 Japan aut NATIONALLICENCE 700 1- Hiramatsu Yoshio National Institute of Animal Health, Tsukuba Science City, Ibaraki, 305 Japan, 1263-2 Nishigata, Niimi, Okayama, 718 Japan, 2-5-1 Shikatacho, Okayama, 700 Japan aut NATIONALLICENCE 700 1- Uno Fumio Niimi Women's College 1263-2 Nishigata, Niimi, Okayama, 718 Japan aut NATIONALLICENCE 700 1- Nii Shiro National Institute of Animal Health, Tsukuba Science City, Ibaraki, 305 Japan, 1263-2 Nishigata, Niimi, Okayama, 718 Japan, 2-5-1 Shikatacho, Okayama, 700 Japan aut NATIONALLICENCE 773 0- Microscopy Oxford University Press 45/3(1996-06), 223-231 0022-0744 45:3<223 1996 45 jmicro Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford