caa a22 4500 397570333 CHVBK 20180308164839.0 cr unu---uuuuu 161202e199606 xx s 000 0 eng 10.1093/oxfordjournals.jmicro.a023434 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jmicro.a023434 WGA Lectin Binding Sites of the Apical Surface of Corti Epithelium: Enhancement by Back-Scattered Electron Imaging in Guinea-Pig Inner Ear [Elektronische Daten] [Yukio Katori, Akira Tonosaki, Tomonori Takasaka] The apical surface of the guinea-pig organ of Corti was examined with SEM after WGA-lectin histochemistry which has a binding specificity for sialic acid and N-acetyl-glucosamine. The contrast of colloidal gold markers were particularly enhanced by back-scattered electron imaging. WGA lectin showed remarkable preference for the microvilli as well as flat surface areas in the supporting cells. Stereociliary surfaces and interstereociliary connections of the sensory hair cell were strongly labelled, whilst only rarely the markers were found in the flat areas of the apical surface of the hair cell. The connections, which are known to be involved in the initiation of the mechanoelectrical transduction, should consist of sialic acid and/or N-acetyl-glucosamine. © 1996 Oxford University Press Full-length Papers nationallicence Corti epithelium nationallicence hair cell nationallicence WGA lectin nationallicence SEM nationallicence back-scattered electron imaging nationallicence Katori Yukio Department of Otolaryngology, Tohoku University School of Medicine Sendai, 980 Japan aut Tonosaki Akira Department of Anatomy, Yamagata University School of Medicine Yamagata, 990-23 Japan aut Takasaka Tomonori Department of Otolaryngology, Tohoku University School of Medicine Sendai, 980 Japan aut Microscopy Oxford University Press 45/3(1996-06), 207-212 0022-0744 45:3<207 1996 45 jmicro https://doi.org/10.1093/oxfordjournals.jmicro.a023434 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jmicro.a023434 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Katori Yukio Department of Otolaryngology, Tohoku University School of Medicine Sendai, 980 Japan aut NATIONALLICENCE 700 1- Tonosaki Akira Department of Anatomy, Yamagata University School of Medicine Yamagata, 990-23 Japan aut NATIONALLICENCE 700 1- Takasaka Tomonori Department of Otolaryngology, Tohoku University School of Medicine Sendai, 980 Japan aut NATIONALLICENCE 773 0- Microscopy Oxford University Press 45/3(1996-06), 207-212 0022-0744 45:3<207 1996 45 jmicro Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford