caa a22 4500 397572131 CHVBK 20180308164844.0 cr unu---uuuuu 161202e19960601xx s 000 0 eng 10.1093/ee/25.3.659 doi (NATIONALLICENCE)oxford-10.1093/ee/25.3.659 Insect Bioassay for Determining Soil Degradation of Bacillus thuringiensis subsp. kurstaki CryIA(b) Protein in Corn Tissue [Elektronische Daten] [Steven R. Sims, Larry R. Holden] A bioassay using larval growth inhibition of Heliothis virescens (F.) was used to study the environmental fate (=soil degradation) of Bacillus thuringiensis subsp. kurstaki CryIA(b) protein in transgenic corn plant tissue (transgenic com). Transgenic com was incubated for up to 43 d, with and without contact with soil, in a chamber maintaining warm (24-2°C) and humid (>80% RH) conditions. Incubation was terminated by moving the transgenic com to −8O°C. A series of dilutions was made from each incubated sample, mixed into artificial insect diet, and fed to neonate H. virescens. Weights of treated larvae were scored after 7 d growth at ≍28°C, and the bioactivity of CryIA(b) protein in the samples was determined using regression analysis of the dose-weight response data. The insect bioassay approach allowed for nearly complete recovery of CryIA(b) protein bioactivity in a transgenic corn + soil matrix or transgenic com incubated without soil contact. CryIA(b) protein added to soil as a component of transgenic com tissue had an estimated DT50(50° dissipation time = ‘half-life”) of 1.6 d and a DT90 (90% dissipation time) of 15 d. CryIA(b) protein in transgenic com tissue incubated without soil contact, had an estimated DT50 of 25.6 d and a DT90 of 40.7 d. The results suggest that CryIA(b) protein, as a component of postharvest transgenic corn plants, will dissipate readily on the surface of, or cultivated into, soil. © 1996 Entomological Society of America Physiological and Chemical Ecolocy nationallicence Heliothis virescens nationallicence transgenic com nationallicence CryIA(b) protein nationallicence soil degradation nationallicence Sims Steven R. Ceregen, a Unit of Monsanto Company, 700 Chesterfield Parkway North, Chesterfield, MO 63198 aut Holden Larry R. Ceregen, a Unit of Monsanto Company, 700 Chesterfield Parkway North, Chesterfield, MO 63198 aut Environmental Entomology Oxford University Press 25/3(1996-06-01), 659-664 0046-225X 25:3<659 1996 25 ee https://doi.org/10.1093/ee/25.3.659 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/ee/25.3.659 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sims Steven R. Ceregen, a Unit of Monsanto Company, 700 Chesterfield Parkway North, Chesterfield, MO 63198 aut NATIONALLICENCE 700 1- Holden Larry R. Ceregen, a Unit of Monsanto Company, 700 Chesterfield Parkway North, Chesterfield, MO 63198 aut NATIONALLICENCE 773 0- Environmental Entomology Oxford University Press 25/3(1996-06-01), 659-664 0046-225X 25:3<659 1996 25 ee Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford