caa a22 4500 397573588 CHVBK 20180308164848.0 cr unu---uuuuu 161202e199608 xx s 000 0 eng 10.1093/hmg/5.8.1083 doi (NATIONALLICENCE)oxford-10.1093/hmg/5.8.1083 The Fragile X Mental Retardation Protein is a Ribonucleoprotein Containing Both Nuclear Localization and Nuclear Export Signals [Elektronische Daten] [Derek E. Eberhart, Henry E. Malter, Yue Feng, Stephen T. Warren] Fragile X syndrome is a frequent cause of mental retardation resulting from the absence of FMRP, the protein encoded by the FMR1 gene. FMRP is an RNA-binding protein of unknown function which is associated with ribosomes. To gain insight into FMRP function, we performed immunolocalization analysis of FMRP truncation and fusion constructs which revealed a nuclear localization signal (NLS) in the amino terminus of FMRP as well as a nuclear export signal (NES) encoded by exon 14. A 17 amino acid peptide containing the FMRP NES, which closely resembles the NES motifs recently described for HIV-1 Rev and PKI, is sufficient to direct nuclear export of a microinjected protein conjugate. Sucrose gradient analysis shows that FMRP ribosome association is RNA-dependent and FMRP is found in ribonucleoprotein (RNP) particles following EDTA treatment. These data are consistent with nascent FMRP entering the nucleus to assemble into mRNP particles prior to export back into the cytoplasm and suggests that fragile X syndrome may result from altered translation of transcripts which normally bind to FMRP. © 1996 Oxford University Press Eberhart Derek E. Howard Hughes Medical Institute and Departments of Biochemistry and Pediatrics, Emory University School of Medicine, Atlanta, Georgia 30322, USA aut Malter Henry E. Howard Hughes Medical Institute and Departments of Biochemistry and Pediatrics, Emory University School of Medicine, Atlanta, Georgia 30322, USA aut Feng Yue Howard Hughes Medical Institute and Departments of Biochemistry and Pediatrics, Emory University School of Medicine, Atlanta, Georgia 30322, USA aut Warren Stephen T. Howard Hughes Medical Institute and Departments of Biochemistry and Pediatrics, Emory University School of Medicine, Atlanta, Georgia 30322, USA aut Human Molecular Genetics Oxford University Press 5/8(1996-08), 1083-1091 0964-6906 5:8<1083 1996 5 hmg https://doi.org/10.1093/hmg/5.8.1083 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/hmg/5.8.1083 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Eberhart Derek E. Howard Hughes Medical Institute and Departments of Biochemistry and Pediatrics, Emory University School of Medicine, Atlanta, Georgia 30322, USA aut NATIONALLICENCE 700 1- Malter Henry E. Howard Hughes Medical Institute and Departments of Biochemistry and Pediatrics, Emory University School of Medicine, Atlanta, Georgia 30322, USA aut NATIONALLICENCE 700 1- Feng Yue Howard Hughes Medical Institute and Departments of Biochemistry and Pediatrics, Emory University School of Medicine, Atlanta, Georgia 30322, USA aut NATIONALLICENCE 700 1- Warren Stephen T. Howard Hughes Medical Institute and Departments of Biochemistry and Pediatrics, Emory University School of Medicine, Atlanta, Georgia 30322, USA aut NATIONALLICENCE 773 0- Human Molecular Genetics Oxford University Press 5/8(1996-08), 1083-1091 0964-6906 5:8<1083 1996 5 hmg Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford