caa a22 4500 397573634 CHVBK 20180308164848.0 cr unu---uuuuu 161202e199610 xx s 000 0 eng 10.1093/hmg/5.10.1559 doi (NATIONALLICENCE)oxford-10.1093/hmg/5.10.1559 Expression of the dystrophin isoform Dp71 in differentiating human fetal myogenic cultures [Elektronische Daten] [Christine N. Tennyson, Ghassan Y. Dally, Peter N. Ray, Ronald G. Worton] The dystrophin gene defective in Duchenne muscular dystrophy (DMD) is extreme in size and complexity with several promoters which direct expression of different isoforms in different tissues. In contrast with adult skeletal muscle which expresses 427 kDa dystrophin, fetal muscle tissue expresses the 71 kDa ubiquitous isoform Dp71 as well as 427 kDa muscle dystrophin. To examine Dp71 expression in fetal muscle further, we have monitored its expression pattern in differentiating myogenic cultures of human fetal muscle origin. The presence of transcripts initiated from the Dp71 promoter was demonstrated by quantitative RT-PCR. The level of transcript expressed from the Dp71 promoter did not change significantly during myogenic differentiation, consistent with the housekeeping nature of the promoter. Measurements to determine the stability of the Dp71 mRNA indicated that it has a half-life of 20 h and, therefore, is somewhat more stable than the larger 14 kb muscle dystrophin mRNA (t½ = 16 h). In contrast with the constant level of Dp71 transcript during myogenic differentiation, the level of Dp71 protein increased significantly, perhaps due to changes in translation efficiency or protein stability. These results demonstrate expression and posttranscriptional upregulation of Dp71 in human fetal myogenic cultures. © 1996 Oxford University Press Tennyson Christine N. Department of Molecular and Medical Genetics, University of Toronto and Genetics Department and Research Institute, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada aut Dally Ghassan Y. Department of Molecular and Medical Genetics, University of Toronto and Genetics Department and Research Institute, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada aut Ray Peter N. Department of Molecular and Medical Genetics, University of Toronto and Genetics Department and Research Institute, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada aut Worton Ronald G. Department of Molecular and Medical Genetics, University of Toronto and Genetics Department and Research Institute, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada aut Human Molecular Genetics Oxford University Press 5/10(1996-10), 1559-1566 0964-6906 5:10<1559 1996 5 hmg https://doi.org/10.1093/hmg/5.10.1559 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/hmg/5.10.1559 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tennyson Christine N. Department of Molecular and Medical Genetics, University of Toronto and Genetics Department and Research Institute, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada aut NATIONALLICENCE 700 1- Dally Ghassan Y. Department of Molecular and Medical Genetics, University of Toronto and Genetics Department and Research Institute, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada aut NATIONALLICENCE 700 1- Ray Peter N. Department of Molecular and Medical Genetics, University of Toronto and Genetics Department and Research Institute, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada aut NATIONALLICENCE 700 1- Worton Ronald G. Department of Molecular and Medical Genetics, University of Toronto and Genetics Department and Research Institute, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada aut NATIONALLICENCE 773 0- Human Molecular Genetics Oxford University Press 5/10(1996-10), 1559-1566 0964-6906 5:10<1559 1996 5 hmg Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford