caa a22 4500 397573685 CHVBK 20180308164848.0 cr unu---uuuuu 161202e199609 xx s 000 0 eng 10.1093/hmg/5.9.1259 doi (NATIONALLICENCE)oxford-10.1093/hmg/5.9.1259 Cloning and Expression Analyses of Mouse Dystroglycan Gene: Specific Expression in Maternal Decidua at the Peri-Implantation Stage [Elektronische Daten] [Shinichi Yotsumoto, Hiroyuki Fujiwara, Joseph H. Horton, Tracy A. Mosby, Xueqian Wang, Yushun Cui, Minoru S. H. Ko] While constructing a catalog of mouse cDNAs which are expressed in the maternal-fetal interface during the peri-implantation period, we encountered a 1.6 kb cDNA clone showing a strong sequence similarity to the 3′ untranslated region of the human dystroglycan gene. We cloned an additional 1.7 kb cDNA by reverse transcriptase-PCR (RT-PCR) and confirmed that this is a true mouse homolog of human dystroglycan cDNA by sequence analyses, Southern blotting, and genetic mapping of this gene on the distal region of mouse chromosome 9. Although it is well established that dystroglycan, a transmembrane protein, plays an important role in muscle tissues by bridging intracellular dystrophin to the laminin in the extracellular matrix, its role in non-muscle tissues remains elusive. To further investigate the role of the dystroglycan gene at the peri-implantation stage, we analyzed the expression patterns of this gene by in situ hybridization, which revealed that this gene is specifically expressed in decidual cells, especially in the cells surrounding the implantation site at 6.5, 7.5, and 8.5 day post conception (p.c.) stages, but not expressed in non-pregnant endometrial cells of uterus nor in the decidua at 12.5 day p.c. Further analyses by RT-PCR confirmed that the amount of dystroglycan mRNA in 8.5 day p.c. decidua was indeed 100-fold higher than that of non-pregnant uterus and 12.5 day p.c. mature placenta. These results suggest that dystroglycan may work as a mediator for adhesion between decidual cells themselves or between decidual cells and trophoblast cells, and provide a structural and functional support for maintaining pregnancy at its early stage. © 1996 Oxford University Press Yotsumoto Shinichi Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, 5047 Gullen Mall, Detroit, Michigan 48202, USA aut Fujiwara Hiroyuki Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, 5047 Gullen Mall, Detroit, Michigan 48202, USA aut Horton Joseph H. Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, 5047 Gullen Mall, Detroit, Michigan 48202, USA aut Mosby Tracy A. Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, 5047 Gullen Mall, Detroit, Michigan 48202, USA aut Wang Xueqian Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, 5047 Gullen Mall, Detroit, Michigan 48202, USA aut Cui Yushun Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, 5047 Gullen Mall, Detroit, Michigan 48202, USA aut Ko Minoru S. H. Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, 5047 Gullen Mall, Detroit, Michigan 48202, USA aut Human Molecular Genetics Oxford University Press 5/9(1996-09), 1259-1267 0964-6906 5:9<1259 1996 5 hmg https://doi.org/10.1093/hmg/5.9.1259 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/hmg/5.9.1259 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yotsumoto Shinichi Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, 5047 Gullen Mall, Detroit, Michigan 48202, USA aut NATIONALLICENCE 700 1- Fujiwara Hiroyuki Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, 5047 Gullen Mall, Detroit, Michigan 48202, USA aut NATIONALLICENCE 700 1- Horton Joseph H. Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, 5047 Gullen Mall, Detroit, Michigan 48202, USA aut NATIONALLICENCE 700 1- Mosby Tracy A. Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, 5047 Gullen Mall, Detroit, Michigan 48202, USA aut NATIONALLICENCE 700 1- Wang Xueqian Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, 5047 Gullen Mall, Detroit, Michigan 48202, USA aut NATIONALLICENCE 700 1- Cui Yushun Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, 5047 Gullen Mall, Detroit, Michigan 48202, USA aut NATIONALLICENCE 700 1- Ko Minoru S. H. Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, 5047 Gullen Mall, Detroit, Michigan 48202, USA aut NATIONALLICENCE 773 0- Human Molecular Genetics Oxford University Press 5/9(1996-09), 1259-1267 0964-6906 5:9<1259 1996 5 hmg Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford