caa a22 4500 397573804 CHVBK 20180308164849.0 cr unu---uuuuu 161202e199611 xx s 000 0 eng 10.1093/hmg/5.11.1703 doi (NATIONALLICENCE)oxford-10.1093/hmg/5.11.1703 Immunology of Gene Therapy with Adenoviral Vectors in Mouse Skeletal Muscle [Elektronische Daten] [Yiping Yang, Sarah Ehlen Haecker, Qin Su, James M. Wilson] Skeletal muscle is an attractive target for somatic gene transfer of both acquired and inherited disorders. Direct injection of adenoviral vectors in the skeletal muscle leads to recombinant gene expression in a large number of muscle fibers. Transgene expression has been transient in most organs and associated with substantial inflammation when experiments are performed in adult immune competent mice. In this report, we utilize a variety ofn vivo and in vitro models of T and B cell function to characterize the nature of the immune response to adenoviral vectors injected into murine skeletal muscle. Cellular immunity dependent on CD4+ and CD8+ T cells contributes to the loss of recombinant gene expression and the development of localized inflammation. Antigen specific activation of T cells occurs to both viral proteins and the reporter gene β-galactosidase. Systemic levels of neutralizing antibody to the capsid proteins of the vector are also generated. Destructive immune responses responsible for loss of transgene expression are largely directed against β-galactosidase in that transgene expression was stable whenβ-galactosidase was eliminated as a neoantigen in mice transgenic forlacZ A strategy to prevent the cellular and humoral immunity to this therapy was developed based on transiently ablating CD4+ T cell activation at the time of vector delivery. Encouraging results were obtained when vector was administered with one of several immune modulating agents including cyclophosphamide, mAb to CD4 cells, and mAb to CD40 ligand. These studies indicate that cellular and humoral immune responses are elicited in the context of gene therapy directed to skeletal muscle with adenoviral vectors. Transient ablation of CD4 T cell activation prevents the effector responses of the CD8 T and B cells. © 1996 Oxford University Press Yang Yiping Institute for Human Gene Therapy, and Department of Molecular and Cellular Engineering, The University of Pennsylvania Medical Center, and the Wistar Institute, Philadelphia, PA 19104, USA aut Ehlen Haecker Sarah Institute for Human Gene Therapy, and Department of Molecular and Cellular Engineering, The University of Pennsylvania Medical Center, and the Wistar Institute, Philadelphia, PA 19104, USA aut Su Qin Institute for Human Gene Therapy, and Department of Molecular and Cellular Engineering, The University of Pennsylvania Medical Center, and the Wistar Institute, Philadelphia, PA 19104, USA aut Wilson James M. Institute for Human Gene Therapy, and Department of Molecular and Cellular Engineering, The University of Pennsylvania Medical Center, and the Wistar Institute, Philadelphia, PA 19104, USA aut Human Molecular Genetics Oxford University Press 5/11(1996-11), 1703-1712 0964-6906 5:11<1703 1996 5 hmg https://doi.org/10.1093/hmg/5.11.1703 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/hmg/5.11.1703 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Yiping Institute for Human Gene Therapy, and Department of Molecular and Cellular Engineering, The University of Pennsylvania Medical Center, and the Wistar Institute, Philadelphia, PA 19104, USA aut NATIONALLICENCE 700 1- Ehlen Haecker Sarah Institute for Human Gene Therapy, and Department of Molecular and Cellular Engineering, The University of Pennsylvania Medical Center, and the Wistar Institute, Philadelphia, PA 19104, USA aut NATIONALLICENCE 700 1- Su Qin Institute for Human Gene Therapy, and Department of Molecular and Cellular Engineering, The University of Pennsylvania Medical Center, and the Wistar Institute, Philadelphia, PA 19104, USA aut NATIONALLICENCE 700 1- Wilson James M. Institute for Human Gene Therapy, and Department of Molecular and Cellular Engineering, The University of Pennsylvania Medical Center, and the Wistar Institute, Philadelphia, PA 19104, USA aut NATIONALLICENCE 773 0- Human Molecular Genetics Oxford University Press 5/11(1996-11), 1703-1712 0964-6906 5:11<1703 1996 5 hmg Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford