caa a22 4500 397573936 CHVBK 20180308164849.0 cr unu---uuuuu 161202e199605 xx s 000 0 eng 10.1093/hmg/5.5.595 doi (NATIONALLICENCE)oxford-10.1093/hmg/5.5.595 Isolation of Genes Amplified in Human Cancers by Microdissection Mediated cDNA Capture [Elektronische Daten] [Edgardo Gracia, Ulrike Fischer, Abdel ElKahloun, Jeffrey M. Trent, Eckart Meese, Paul S. Meltzer] It has been increasingly recognized that homogeneously staining regions (hsr) in human cancers may be complex structures composed of large amplified DNA domains containing multiple genes. It is therefore important to devise strategies for the rapid isolation of cDNAs expressed from these structures. Using a procedure we term microdissection mediated cDNA capture, we recovered hsr specific cDNAs from two different human tumors. The glioblastoma cell line TX3868 and the human sarcoma cell line OsA-CL carry hsrs containing amplified sequences from chromosome 12q13-15. We recovered 17 hsr specific cDNAs following microdissection of these hsrs which had been previously hybridized in situ with linkered cDNA. Northern blot analysis with these cDNAs revealed hybridization to distinct transcripts in OsA-CL RNA and TX3868 RNA. None of the OsA-CL cDNA clones showed cross hybridization with the TX3868 cDNAs suggesting that despite their coincident band localization on 12q, the OsA-CL and TX3868 amplification units do not completely overlap. These results significantly increase the number of amplified genes assigned to the 12q13-15 amplicon illustrating both the complexity of hsrs derived from this region and the utility of microdissection mediated cDNA capture to gain rapid access to cDNAs transcribed from amplified genes. © 1996 Oxford University Press Gracia Edgardo Laboratory of Cancer Genetics, National Center for Human Genome Research, National Institutes of Health, Bethesda Maryland 20892, USA aut Fischer Ulrike Institutfur Humangenetik, Universitatskliniken des Saarlandes, Homburg/Saar, Germany aut ElKahloun Abdel Laboratory of Cancer Genetics, National Center for Human Genome Research, National Institutes of Health, Bethesda Maryland 20892, USA aut Trent Jeffrey M. Laboratory of Cancer Genetics, National Center for Human Genome Research, National Institutes of Health, Bethesda Maryland 20892, USA aut Meese Eckart Institutfur Humangenetik, Universitatskliniken des Saarlandes, Homburg/Saar, Germany aut Meltzer Paul S. Laboratory of Cancer Genetics, National Center for Human Genome Research, National Institutes of Health, Bethesda Maryland 20892, USA aut Human Molecular Genetics Oxford University Press 5/5(1996-05), 595-600 0964-6906 5:5<595 1996 5 hmg https://doi.org/10.1093/hmg/5.5.595 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/hmg/5.5.595 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gracia Edgardo Laboratory of Cancer Genetics, National Center for Human Genome Research, National Institutes of Health, Bethesda Maryland 20892, USA aut NATIONALLICENCE 700 1- Fischer Ulrike Institutfur Humangenetik, Universitatskliniken des Saarlandes, Homburg/Saar, Germany aut NATIONALLICENCE 700 1- ElKahloun Abdel Laboratory of Cancer Genetics, National Center for Human Genome Research, National Institutes of Health, Bethesda Maryland 20892, USA aut NATIONALLICENCE 700 1- Trent Jeffrey M. Laboratory of Cancer Genetics, National Center for Human Genome Research, National Institutes of Health, Bethesda Maryland 20892, USA aut NATIONALLICENCE 700 1- Meese Eckart Institutfur Humangenetik, Universitatskliniken des Saarlandes, Homburg/Saar, Germany aut NATIONALLICENCE 700 1- Meltzer Paul S. Laboratory of Cancer Genetics, National Center for Human Genome Research, National Institutes of Health, Bethesda Maryland 20892, USA aut NATIONALLICENCE 773 0- Human Molecular Genetics Oxford University Press 5/5(1996-05), 595-600 0964-6906 5:5<595 1996 5 hmg Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford