caa a22 4500 397574061 CHVBK 20180308164850.0 cr unu---uuuuu 161202e199612 xx s 000 0 eng 10.1093/hmg/5.12.2005 doi (NATIONALLICENCE)oxford-10.1093/hmg/5.12.2005 A SPGY Copy Homologous to the Mouse Gene Dazla and the Drosophila Gene Boule is Autosomal and Expressed Only in the Human Male Gonad [Elektronische Daten] [Zhihong Shan, Peter Hirschmann, Thomas Seebacher, Angela Edelmann, Anna Jauch, Jane Morell, Peter Urbitsch, Peter H. Vogt] We have isolated a series of human testis poly(A) cDNA clones by cross-hybridization to SPGY1, a Y gene homologous to DAZ. Their sequence analysis revealed an identical nucleotide composition in different ‘full-length' clones, suggesting that all were encoded by the same gene. We mapped this gene to the short arm of chromosome 3 and designated it SPGYLA (SPGY like autosomal). Comparison of the SPGYLA cDNA sequence with the cDNA sequences of DAZ and SPGY1 revealed two prominent differences. The tandem repetitive structure of 72 bp sequence units (DAZ repeats) is absent. SPGYLA contains only one 72 bp sequence unit. Downstream of it, a specific 130 bp sequence domain is present which is absent in DAZ and SPGY1 but present in the mouse gene Dazla and in the Drosophila gene boule. SPGYLA encodes an RNA binding protein expressed only in the human male gonad. The data presented give strong evidence that not DAZ but SPGYLA is the functional human homologue of Dazla and boule. © 1993 Oxford University Press Shan Zhihong Reproductiongenetics in Institute of Human Genetics, University of Heidelberg, D-69120 Heidelberg, Germany aut Hirschmann Peter Reproductiongenetics in Institute of Human Genetics, University of Heidelberg, D-69120 Heidelberg, Germany aut Seebacher Thomas Reproductiongenetics in Institute of Human Genetics, University of Heidelberg, D-69120 Heidelberg, Germany aut Edelmann Angela Reproductiongenetics in Institute of Human Genetics, University of Heidelberg, D-69120 Heidelberg, Germany aut Jauch Anna Reproductiongenetics in Institute of Human Genetics, University of Heidelberg, D-69120 Heidelberg, Germany aut Morell Jane Department Reproductionbiology, German Primate Center GmbH, D-37077 Göttingen, Germany aut Urbitsch Peter Reproductiongenetics in Institute of Human Genetics, University of Heidelberg, D-69120 Heidelberg, Germany aut Vogt Peter H. Reproductiongenetics in Institute of Human Genetics, University of Heidelberg, D-69120 Heidelberg, Germany aut Human Molecular Genetics Oxford University Press 5/12(1996-12), 2005-2011 0964-6906 5:12<2005 1996 5 hmg https://doi.org/10.1093/hmg/5.12.2005 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/hmg/5.12.2005 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shan Zhihong Reproductiongenetics in Institute of Human Genetics, University of Heidelberg, D-69120 Heidelberg, Germany aut NATIONALLICENCE 700 1- Hirschmann Peter Reproductiongenetics in Institute of Human Genetics, University of Heidelberg, D-69120 Heidelberg, Germany aut NATIONALLICENCE 700 1- Seebacher Thomas Reproductiongenetics in Institute of Human Genetics, University of Heidelberg, D-69120 Heidelberg, Germany aut NATIONALLICENCE 700 1- Edelmann Angela Reproductiongenetics in Institute of Human Genetics, University of Heidelberg, D-69120 Heidelberg, Germany aut NATIONALLICENCE 700 1- Jauch Anna Reproductiongenetics in Institute of Human Genetics, University of Heidelberg, D-69120 Heidelberg, Germany aut NATIONALLICENCE 700 1- Morell Jane Department Reproductionbiology, German Primate Center GmbH, D-37077 Göttingen, Germany aut NATIONALLICENCE 700 1- Urbitsch Peter Reproductiongenetics in Institute of Human Genetics, University of Heidelberg, D-69120 Heidelberg, Germany aut NATIONALLICENCE 700 1- Vogt Peter H. Reproductiongenetics in Institute of Human Genetics, University of Heidelberg, D-69120 Heidelberg, Germany aut NATIONALLICENCE 773 0- Human Molecular Genetics Oxford University Press 5/12(1996-12), 2005-2011 0964-6906 5:12<2005 1996 5 hmg Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford