caa a22 4500 39757407X CHVBK 20180308164850.0 cr unu---uuuuu 161202e199609 xx s 000 0 eng 10.1093/hmg/5.Supplement_1.1437 doi (NATIONALLICENCE)oxford-10.1093/hmg/5.Supplement_1.1437 Elgar Greg Molecular Genetics, Dept. of Medicine, University of Cambridge, Level 5, Addenbrookes' Hospital, Hills Road, Cambridge CB2 2QQ, UK Quality not quantity: the pufferfish genome [Elektronische Daten] [Greg Elgar] The genome of the pufferfish, Fugu rubripes (Fugu) is compact. With a similar gene complement to mammals and a genome size of just 400 Mb, gene density is high averaging one every 6-7 kb. Initial characterization of this genome has shown that although genes are much smaller and more densely spaced, their intron/exon structure is conserved with the resulting introns being small. There is little repetitive DNA in the genome and this greatly facilitates comparative genomic studies. The coding content of genes is highly conserved as are critical regulatory elements of some genes. Other DNA is not, however, and this allows the identification of homologous coding sequence between Fugu and mammalian genes. Although the genome of Fugu is 7.5 times smaller than the human genome, not all genes are reduced proportionately. Some regions of the genome show conserved synteny with mammalian genomes, although at the present time only short physical distances have been examined. The structure of the genome is also being studied. Initial data suggest that this may be different to that found in mammals. It is not clear that the same kind of isochore structure is present in this early vertebrate genome. Patterns of methylation may be different resulting in a different distribution of CpG islands. An attempt is being made to centralize both resources and data from the genome of Fugu so that everything may be integrated into a single, publicly accessible database which in turn, may be integrated with databases from other organisms. © 1996 Oxford University Press Human Molecular Genetics Oxford University Press 5/Supplement_1(1996-09), 1437-1442 0964-6906 5:Supplement_1<1437 1996 5 hmg https://doi.org/10.1093/hmg/5.Supplement_1.1437 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/hmg/5.Supplement_1.1437 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Elgar Greg Molecular Genetics, Dept. of Medicine, University of Cambridge, Level 5, Addenbrookes' Hospital, Hills Road, Cambridge CB2 2QQ, UK NATIONALLICENCE 773 0- Human Molecular Genetics Oxford University Press 5/Supplement_1(1996-09), 1437-1442 0964-6906 5:Supplement_1<1437 1996 5 hmg Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford