caa a22 4500 397574584 CHVBK 20180308164851.0 cr unu---uuuuu 161202e199603 xx s 000 0 eng 10.1093/hmg/5.3.381 doi (NATIONALLICENCE)oxford-10.1093/hmg/5.3.381 cDNA Cloning and Expression of rsca1, the Rat Counterpart of the Human Spinocerebellar Ataxia Type 1 Gene [Elektronische Daten] [Martin Gossen, Ina Schmitt, Kirstin Obst, Petra Wahle, Jörg T. Epplen, Olaf Riess] Spinocerebellar ataxia type 1 (SCA1) is a neurodegenerative disorder caused by an expanded and unstable (CAG)>40 repeat within a gene of unknown function. We isolated the complete coding region of the rat SCA1 gene (rsca1), the 5′-untranslated region (UTR) and 1.3 kb of the 3′-UTR. The rat sequence exhibits 90% peptide identity to the human counterpart. In comparison to human, the rat (CAG)n block is reduced to two trinucleotide motifs preceded by three different proline codons not present in man. Furthermore, we investigated the expression of rsca1 in different rat tissues. The rsca1 gene is predominantly expressed in brain throughout all developmental stages. In situ hybridizations reveal high levels of expression in various regions of the adult rat brain, including cerebellum, hippocampus and cortex. © 1996 Oxford University Press Gossen Martin Molecular Human Genetics, Ruhr-University, Bochum 44780, Germany aut Schmitt Ina Molecular Human Genetics, Ruhr-University, Bochum 44780, Germany aut Obst Kirstin Department of Zoology and Neurobiology, Ruhr-University, Bochum 44780, Germany aut Wahle Petra Department of Zoology and Neurobiology, Ruhr-University, Bochum 44780, Germany aut Epplen Jörg T. Molecular Human Genetics, Ruhr-University, Bochum 44780, Germany aut Riess Olaf Molecular Human Genetics, Ruhr-University, Bochum 44780, Germany aut Human Molecular Genetics Oxford University Press 5/3(1996-03), 381-389 0964-6906 5:3<381 1996 5 hmg https://doi.org/10.1093/hmg/5.3.381 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/hmg/5.3.381 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gossen Martin Molecular Human Genetics, Ruhr-University, Bochum 44780, Germany aut NATIONALLICENCE 700 1- Schmitt Ina Molecular Human Genetics, Ruhr-University, Bochum 44780, Germany aut NATIONALLICENCE 700 1- Obst Kirstin Department of Zoology and Neurobiology, Ruhr-University, Bochum 44780, Germany aut NATIONALLICENCE 700 1- Wahle Petra Department of Zoology and Neurobiology, Ruhr-University, Bochum 44780, Germany aut NATIONALLICENCE 700 1- Epplen Jörg T. Molecular Human Genetics, Ruhr-University, Bochum 44780, Germany aut NATIONALLICENCE 700 1- Riess Olaf Molecular Human Genetics, Ruhr-University, Bochum 44780, Germany aut NATIONALLICENCE 773 0- Human Molecular Genetics Oxford University Press 5/3(1996-03), 381-389 0964-6906 5:3<381 1996 5 hmg Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford