caa a22 4500 397574614 CHVBK 20180308164851.0 cr unu---uuuuu 161202e199604 xx s 000 0 eng 10.1093/hmg/5.4.423 doi (NATIONALLICENCE)oxford-10.1093/hmg/5.4.423 Characterization of a Cluster of Sulfatase Genes on Xp22.3 Suggests Gene Duplications in an Ancestral Pseudoautosomal Region [Elektronische Daten] [Germana Meroni, Brunella Franco, Nicoletta Archidiacono, Silvia Messali, Grazia Andolfi, Mariano Rocchi, Andrea Ballabio] An obligatory crossing-over event between the X and Y chromosomes in mammals occurs at each male meiosis within the 2.6 Mb of DNA defining the pseudoautosomal region (PAR). Genes located within or near the human PAR have homologous copies on the X and Y chromosomes, escape X inactivation and appear to be highly divergent throughout evolution. We have characterized the genomic structure of two genes from a recently identified cluster of sulfatase genes (ARSD and ARSE) located in the Xp22.3 region, and of their homologs on the Y chromosome. Our results indicate that the ARSD and ARSE genes from within this cluster have a conserved genomic organization, shared also by another Xp22.3 gene, STS, but completely different from that of all the other sulfatase genes. Sequence analysis of the Y-linked homologs indicate that they represent truncated pseudogenes. Sequence identity values between the X and Y copies of each gene is on average 91%, significantly higher than the values obtained by comparing different members of the family. FISH mapping experiments performed in several primate species revealed an identical localization of the X-linked copies to that in man, but different localizations of the Y homologs. Together, our data indicate that the cluster of sulfatase genes on human Xp22.3 was created through duplication events which probably occurred in an ancestral PAR, and support the view that the PAR has undergone multiple changes during recent mammalian evolution. © 1996 Oxford University Press Meroni Germana Telethon Institute of Genetics and Medicine (TIGEM), San Raffaele Biomedical Science Park, Milano, Italy aut Franco Brunella Telethon Institute of Genetics and Medicine (TIGEM), San Raffaele Biomedical Science Park, Milano, Italy aut Archidiacono Nicoletta Institute of Genetics, University of Bari, Bari, Italy aut Messali Silvia Telethon Institute of Genetics and Medicine (TIGEM), San Raffaele Biomedical Science Park, Milano, Italy aut Andolfi Grazia Telethon Institute of Genetics and Medicine (TIGEM), San Raffaele Biomedical Science Park, Milano, Italy aut Rocchi Mariano Institute of Genetics, University of Bari, Bari, Italy aut Ballabio Andrea Telethon Institute of Genetics and Medicine (TIGEM), San Raffaele Biomedical Science Park, Milano, Italy aut Human Molecular Genetics Oxford University Press 5/4(1996-04), 423-431 0964-6906 5:4<423 1996 5 hmg https://doi.org/10.1093/hmg/5.4.423 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/hmg/5.4.423 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Meroni Germana Telethon Institute of Genetics and Medicine (TIGEM), San Raffaele Biomedical Science Park, Milano, Italy aut NATIONALLICENCE 700 1- Franco Brunella Telethon Institute of Genetics and Medicine (TIGEM), San Raffaele Biomedical Science Park, Milano, Italy aut NATIONALLICENCE 700 1- Archidiacono Nicoletta Institute of Genetics, University of Bari, Bari, Italy aut NATIONALLICENCE 700 1- Messali Silvia Telethon Institute of Genetics and Medicine (TIGEM), San Raffaele Biomedical Science Park, Milano, Italy aut NATIONALLICENCE 700 1- Andolfi Grazia Telethon Institute of Genetics and Medicine (TIGEM), San Raffaele Biomedical Science Park, Milano, Italy aut NATIONALLICENCE 700 1- Rocchi Mariano Institute of Genetics, University of Bari, Bari, Italy aut NATIONALLICENCE 700 1- Ballabio Andrea Telethon Institute of Genetics and Medicine (TIGEM), San Raffaele Biomedical Science Park, Milano, Italy aut NATIONALLICENCE 773 0- Human Molecular Genetics Oxford University Press 5/4(1996-04), 423-431 0964-6906 5:4<423 1996 5 hmg Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford