caa a22 4500 397574908 CHVBK 20180308164852.0 cr unu---uuuuu 161202e199610 xx s 000 0 eng 10.1093/hmg/5.10.1631 doi (NATIONALLICENCE)oxford-10.1093/hmg/5.10.1631 A High Resolution CEPH Crossover Mapping Panel and Integrated Map of Chromosome 11 [Elektronische Daten] [Pamela R. Fain, Edward N. Kort, Cherine Yousry, Michael R. James, Michael Litt] High resolution (0.1 cM) CEPH crossover mapping panels were constructed for chromosome 11. These panels will facilitate a transition from top-down physical and genetic mapping strategies to integrated breakpoint mapping strategies. Novel methods, which differ from other methods in overcoming the limitations of incomplete heterozygosity and variable marker density, were developed for creating the panels and integrated maps. This made it possible to identify and sublocalize the majority of crossovers in 61 families. The panels were used to map 139 microsatellite markers. A semi-integrated map and a fully-integrated map were constructed by combining these data with data from CEPH 7.1 and then integrating data from the radiation hybrid (RH) map. Genetic lengths estimated from the mapping panels were similar to the estimates obtained when all recombinant and non-recombinant offspring were included (189.4 cM in females and 126.1 cM in males), indicating that genetic distances are stable at this high marker density. The maps have a cM density of 0.62. The distance between ordered markers is 1.39-2.92 cM depending on the criterion for order and the extent of map integration. The 2D maps provide the resolution and flexibility needed to enhance current applications such as positional cloning and mapping complex disorders; while the mapping panels will greatly improve the resolution, reliability and efficiency of future genetic mapping. © 1996 Oxford University Press Fain Pamela R. Department of Medicine, University of Colorado Health Sciences Center, Denver, CO 80262, USA aut Kort Edward N. Division of Genetic Epidemiology, University of Utah, Salt Lake City, UT 84108, USA aut Yousry Cherine Wellcome Trust Centre for Human Genetics, Headington, Oxford, UK aut James Michael R. Wellcome Trust Centre for Human Genetics, Headington, Oxford, UK aut Litt Michael Department of Biochemistry and Medical Genetics, Oregon Health Sciences University, Portland, OR 97201, USA aut Human Molecular Genetics Oxford University Press 5/10(1996-10), 1631-1636 0964-6906 5:10<1631 1996 5 hmg https://doi.org/10.1093/hmg/5.10.1631 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/hmg/5.10.1631 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fain Pamela R. Department of Medicine, University of Colorado Health Sciences Center, Denver, CO 80262, USA aut NATIONALLICENCE 700 1- Kort Edward N. Division of Genetic Epidemiology, University of Utah, Salt Lake City, UT 84108, USA aut NATIONALLICENCE 700 1- Yousry Cherine Wellcome Trust Centre for Human Genetics, Headington, Oxford, UK aut NATIONALLICENCE 700 1- James Michael R. Wellcome Trust Centre for Human Genetics, Headington, Oxford, UK aut NATIONALLICENCE 700 1- Litt Michael Department of Biochemistry and Medical Genetics, Oregon Health Sciences University, Portland, OR 97201, USA aut NATIONALLICENCE 773 0- Human Molecular Genetics Oxford University Press 5/10(1996-10), 1631-1636 0964-6906 5:10<1631 1996 5 hmg Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford