caa a22 4500 397575114 CHVBK 20180308164853.0 cr unu---uuuuu 161202e199607 xx s 000 0 eng 10.1093/hmg/5.7.875 doi (NATIONALLICENCE)oxford-10.1093/hmg/5.7.875 A Model for Testing Recombinogenic Sequences in the Mouse Germline [Elektronische Daten] [Mary Ellen Moynahan, Ercan Akgün, Maria Jasin] Homologous recombination is a conserved process of genetic exchange generated by homologous pairing of nucleotides. Species diversity and gene evolution are dependent on the outcomes of recombination during germ cell development, yet systems to study mammalian germline recombination, especially those with applications to human genetics, are not well developed. We report on a transgenic mouse system designed to study recombination within test sequences in the male germline utilizing an intron-interrupted lacZ reporter gene. β-galactosidase positive sperm are detected and quantitated by flow cytometry using fluorogenic substrates. Examination of recombination within a 1.7 kb repeat of test sequences derived from the human glycophorin breakpoint cluster region detects approximately 0.04-0.09% fluorescent sperm. Confirmation that these sperm result from recombination in the germline comes from histochemical staining of testicular cells, examination of spliced mRNA, and PCR analysis of sorted sperm populations. The system is readily adaptable to studies of other sequences reported to have elevated levels of recombination, including those implicated in human genetic disease. Investigations of the molecular basis for genomic instability at specific chromosomal locations may yield important insights into mechanisms of chromosomal loss and rearrangements. © 1996 Oxford University Press Moynahan Mary Ellen Department of Medicine, Memorial Sloan-Kettering Cancer Center and Cornell University Graduate School of Medical Sciences, 1275 York Ave, New York, NY 10021, USA aut Akgün Ercan Cell Biology and Genetics Program, Memorial Sloan-Kettering Cancer Center and Cornell University Graduate School of Medical Sciences, 1275 York Ave, New York, NY 10021, USA aut Jasin Maria Cell Biology and Genetics Program, Memorial Sloan-Kettering Cancer Center and Cornell University Graduate School of Medical Sciences, 1275 York Ave, New York, NY 10021, USA aut Human Molecular Genetics Oxford University Press 5/7(1996-07), 875-886 0964-6906 5:7<875 1996 5 hmg https://doi.org/10.1093/hmg/5.7.875 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/hmg/5.7.875 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Moynahan Mary Ellen Department of Medicine, Memorial Sloan-Kettering Cancer Center and Cornell University Graduate School of Medical Sciences, 1275 York Ave, New York, NY 10021, USA aut NATIONALLICENCE 700 1- Akgün Ercan Cell Biology and Genetics Program, Memorial Sloan-Kettering Cancer Center and Cornell University Graduate School of Medical Sciences, 1275 York Ave, New York, NY 10021, USA aut NATIONALLICENCE 700 1- Jasin Maria Cell Biology and Genetics Program, Memorial Sloan-Kettering Cancer Center and Cornell University Graduate School of Medical Sciences, 1275 York Ave, New York, NY 10021, USA aut NATIONALLICENCE 773 0- Human Molecular Genetics Oxford University Press 5/7(1996-07), 875-886 0964-6906 5:7<875 1996 5 hmg Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford