caa a22 4500 397575319 CHVBK 20180308164854.0 cr unu---uuuuu 161202e199609 xx s 000 0 eng 10.1093/hmg/5.9.1207 doi (NATIONALLICENCE)oxford-10.1093/hmg/5.9.1207 Evolutionary Conservation of Sequence and Expression of the bHLH Protein Atonal Suggests a Conserved Role in Neurogenesis [Elektronische Daten] [Nissim Ben-Arie, Alanna E. McCall, Scott Berkman, Gregor Eichele, Hugo J. Bellen, Huda Y. Zoghbi] atonal is a Drosophila proneural gene that belongs to the family of basic helix-loop-helix (bHLH)- containing proteins. It is expressed in the chordotonal organs and photoreceptor cells, and flies that lack Atonal protein are ataxic and blind. Here we report the cloning of atonal homologs from red flour beetle, puffer fish, chicken, mouse, and human. The bHLH domain is conserved throughout evolution, while the entire coding region is highly similar in mammals. Both the chicken and the mouse homologs are expressed early in embryogenesis in the hind brain, and specifically in cells predicted to give rise to the external granular layer of the cerebellum. In addition, these genes are expressed throughout the dorsal part of the spinal cord, in patterns different from those found for other genes, like LH-2 and wnt-1. The mouse homolog (Math1) maps to mouse chromosome 6, and the human homolog (HATH1) to human chromosome 4q22. Two neurological mouse mutants, Lcand chp, were found to map to the vicinity of Math1, but are not caused by mutations in Math1. The evolutionary conservation of this gene and its mRNA expression patterns during embryogenesis suggests that it plays a key role in the development of the vertebrate central nervous system. © 1996 Oxford University Press Ben-Arie Nissim Department of Pediatrics, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA aut McCall Alanna E. Department of Pediatrics, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA aut Berkman Scott Department of Biochemistry, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA aut Eichele Gregor Department of Biochemistry, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA aut Bellen Hugo J. Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA aut Zoghbi Huda Y. Department of Pediatrics, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA aut Human Molecular Genetics Oxford University Press 5/9(1996-09), 1207-1216 0964-6906 5:9<1207 1996 5 hmg https://doi.org/10.1093/hmg/5.9.1207 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/hmg/5.9.1207 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ben-Arie Nissim Department of Pediatrics, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA aut NATIONALLICENCE 700 1- McCall Alanna E. Department of Pediatrics, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA aut NATIONALLICENCE 700 1- Berkman Scott Department of Biochemistry, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA aut NATIONALLICENCE 700 1- Eichele Gregor Department of Biochemistry, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA aut NATIONALLICENCE 700 1- Bellen Hugo J. Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA aut NATIONALLICENCE 700 1- Zoghbi Huda Y. Department of Pediatrics, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA aut NATIONALLICENCE 773 0- Human Molecular Genetics Oxford University Press 5/9(1996-09), 1207-1216 0964-6906 5:9<1207 1996 5 hmg Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford