caa a22 4500 397575408 CHVBK 20180308164854.0 cr unu---uuuuu 161202e199605 xx s 000 0 eng 10.1093/hmg/5.5.685 doi (NATIONALLICENCE)oxford-10.1093/hmg/5.5.685 Heterogeneity in Telomere Length of Human Chromosomes [Elektronische Daten] [Peter M. Lansdorp, Nico P. Verwoerd, Frans M. van de Rijke, Visia Dragowska, Marie-Térèse Little, Roeland W. Dirks, Anton K. Raap, Hans J. Tanke] Vertebrate chromosomes terminate in variable numbers of T2AG3 nucleotide repeats. In order to study telomere repeats at individual chromosomes, we developed novel, quantitative fluorescence in situ hybridization procedures using labeled (C3TA2)3 peptide nucleic acid and digital imaging microscopy. Telomere fluorescence intensity values from metaphase chromosomes of cultured human hematopoietic cells decreased with the replication history of the cells, varied up to six-fold within a metaphase, and were similar between sister chromatid telomeres. Surprisingly, telomere fluorescence intensity values within normal adult bone marrow metaphases did not show a normal distribution, suggesting that a minimum number of repeats at each telomere is required and/or maintained during normal hematopoiesis. © 1996 Oxford University Press Lansdorp Peter M. Terry Fox Laboratory, B.C. Cancer Agency, 601 West 10th Avenue, Vancouver, B.C., V5Z 1L3, Canada aut Verwoerd Nico P. Sylvius Laboratory, Department of Cytochemistry and Cytometry, University of Leiden, Wassenaarseweg 72, 2333 Al Leiden, The Netherlands aut van de Rijke Frans M. Sylvius Laboratory, Department of Cytochemistry and Cytometry, University of Leiden, Wassenaarseweg 72, 2333 Al Leiden, The Netherlands aut Dragowska Visia Terry Fox Laboratory, B.C. Cancer Agency, 601 West 10th Avenue, Vancouver, B.C., V5Z 1L3, Canada aut Little Marie-Térèse Terry Fox Laboratory, B.C. Cancer Agency, 601 West 10th Avenue, Vancouver, B.C., V5Z 1L3, Canada aut Dirks Roeland W. Sylvius Laboratory, Department of Cytochemistry and Cytometry, University of Leiden, Wassenaarseweg 72, 2333 Al Leiden, The Netherlands aut Raap Anton K. Sylvius Laboratory, Department of Cytochemistry and Cytometry, University of Leiden, Wassenaarseweg 72, 2333 Al Leiden, The Netherlands aut Tanke Hans J. Sylvius Laboratory, Department of Cytochemistry and Cytometry, University of Leiden, Wassenaarseweg 72, 2333 Al Leiden, The Netherlands aut Human Molecular Genetics Oxford University Press 5/5(1996-05), 685-691 0964-6906 5:5<685 1996 5 hmg https://doi.org/10.1093/hmg/5.5.685 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/hmg/5.5.685 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lansdorp Peter M. Terry Fox Laboratory, B.C. Cancer Agency, 601 West 10th Avenue, Vancouver, B.C., V5Z 1L3, Canada aut NATIONALLICENCE 700 1- Verwoerd Nico P. Sylvius Laboratory, Department of Cytochemistry and Cytometry, University of Leiden, Wassenaarseweg 72, 2333 Al Leiden, The Netherlands aut NATIONALLICENCE 700 1- van de Rijke Frans M. Sylvius Laboratory, Department of Cytochemistry and Cytometry, University of Leiden, Wassenaarseweg 72, 2333 Al Leiden, The Netherlands aut NATIONALLICENCE 700 1- Dragowska Visia Terry Fox Laboratory, B.C. Cancer Agency, 601 West 10th Avenue, Vancouver, B.C., V5Z 1L3, Canada aut NATIONALLICENCE 700 1- Little Marie-Térèse Terry Fox Laboratory, B.C. Cancer Agency, 601 West 10th Avenue, Vancouver, B.C., V5Z 1L3, Canada aut NATIONALLICENCE 700 1- Dirks Roeland W. Sylvius Laboratory, Department of Cytochemistry and Cytometry, University of Leiden, Wassenaarseweg 72, 2333 Al Leiden, The Netherlands aut NATIONALLICENCE 700 1- Raap Anton K. Sylvius Laboratory, Department of Cytochemistry and Cytometry, University of Leiden, Wassenaarseweg 72, 2333 Al Leiden, The Netherlands aut NATIONALLICENCE 700 1- Tanke Hans J. Sylvius Laboratory, Department of Cytochemistry and Cytometry, University of Leiden, Wassenaarseweg 72, 2333 Al Leiden, The Netherlands aut NATIONALLICENCE 773 0- Human Molecular Genetics Oxford University Press 5/5(1996-05), 685-691 0964-6906 5:5<685 1996 5 hmg Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford