caa a22 4500 397575424 CHVBK 20180308164854.0 cr unu---uuuuu 161202e199603 xx s 000 0 eng 10.1093/hmg/5.3.309 doi (NATIONALLICENCE)oxford-10.1093/hmg/5.3.309 A Human Modifier of Methylation for Class I HLA Genes (Memo-1) Maps to Chromosomal Bands 1p35-36.1 [Elektronische Daten] [N. C. Cheng, A. J. K. Chan, M. M. Beitsma, F. Speleman, A. Westerveld, R. Versteeg] Class I HLA genes are expressed in almost all tissues, but expression is low or undetectable in many neuroblastomas. We analysed class I HLA methylation in normal tissues and in 28 neuroectodermal tumour cell lines. HLA-C is hypermethylated in normal adult tissues and 13 cell lines, while 15 cell lines show the hypomethylated phenotype. Hypomethylation of HLA-C strongly correlates with hemizygous deletion of a 9 cM interval on 1p35-36.1, suggesting that this region encodes a modifier of methylation for HLA-C. To test whether hypomethylation of class I HLA genes results from loss of a modifier gene, we fused a hypomethylating neuroblastoma cell line with a hypermethylating cell line. Methylation of class I HLA genes was induced in the hybrids. Furthermore, methylation of HLA-C, -E and -A genes, which are encoded in a 1.4 Mb region on 6p21, is correlated in most cell lines. Our results suggest that 1p35-36.1 encodes a modifier of methylation for class I HLA genes, that is deleted in many neuroblastomas. © 1996 Oxford University Press Cheng N. C. Department of Human Genetics, University of Amsterdam, Academic Medical Centre, PO Box 22700, 1100 DE Amsterdam, The Netherlands aut Chan A. J. K. Department of Human Genetics, University of Amsterdam, Academic Medical Centre, PO Box 22700, 1100 DE Amsterdam, The Netherlands aut Beitsma M. M. Department of Human Genetics, University of Amsterdam, Academic Medical Centre, PO Box 22700, 1100 DE Amsterdam, The Netherlands aut Speleman F. Department of Medical Genetics, University of Gent, Gent, Belgium aut Westerveld A. Department of Human Genetics, University of Amsterdam, Academic Medical Centre, PO Box 22700, 1100 DE Amsterdam, The Netherlands aut Versteeg R. Department of Human Genetics, University of Amsterdam, Academic Medical Centre, PO Box 22700, 1100 DE Amsterdam, The Netherlands aut Human Molecular Genetics Oxford University Press 5/3(1996-03), 309-317 0964-6906 5:3<309 1996 5 hmg https://doi.org/10.1093/hmg/5.3.309 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/hmg/5.3.309 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cheng N. C. Department of Human Genetics, University of Amsterdam, Academic Medical Centre, PO Box 22700, 1100 DE Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Chan A. J. K. Department of Human Genetics, University of Amsterdam, Academic Medical Centre, PO Box 22700, 1100 DE Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Beitsma M. M. Department of Human Genetics, University of Amsterdam, Academic Medical Centre, PO Box 22700, 1100 DE Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Speleman F. Department of Medical Genetics, University of Gent, Gent, Belgium aut NATIONALLICENCE 700 1- Westerveld A. Department of Human Genetics, University of Amsterdam, Academic Medical Centre, PO Box 22700, 1100 DE Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Versteeg R. Department of Human Genetics, University of Amsterdam, Academic Medical Centre, PO Box 22700, 1100 DE Amsterdam, The Netherlands aut NATIONALLICENCE 773 0- Human Molecular Genetics Oxford University Press 5/3(1996-03), 309-317 0964-6906 5:3<309 1996 5 hmg Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford