caa a22 4500 397575467 CHVBK 20180308164854.0 cr unu---uuuuu 161202e199601 xx s 000 0 eng 10.1093/hmg/5.1.23 doi (NATIONALLICENCE)oxford-10.1093/hmg/5.1.23 Human Pseudoautosomal Boundary-like Sequences: Expression and Involvement in Evolutionary Formation of the Present-day Pseudoautosomal Boundary of Human Sex Chromosomes [Elektronische Daten] [Tatsuo Fukagawa, Yasukazu Nakamura, Katsuzumi Okumura, Masahiro Nogami, Asako Ando, Hidetoshi Inoko, Naruya Saitou, Toshimichi Ikemura] The human genome is composed of long-range mosaic structures of G+C% (GC%), which are thought to be related to chromosome bands. We previously identified a boundary of Mb-level domains of GC% mosaic structures in the human major histocompatibility complex (MHC) and found in the domain boundary a sequence very similar to pseudoautosomal boundary (PAB) sequences of human sex chromosomes. We designated it ‘PABL' and found many PABLs in the human genome. By analysis of six genomic and six transcribed PABLs, a core and consensus sequence of about 650 nt was defined; the 3′-and 5′-edges of the PABLs were strictly conserved. Northern blot analysis showed sizes of PABL transcripts to be 5-10 kb in length. Divergence time of PABLs was estimated to be 60-120 million years ago by analysis of human PABLs and PABXY1 of seven primates, and the evolutionary rates deduced showed PABLs to have been under selective constraints. A model for evolutionary formation of the present pseudoautosomal boundary was proposed by postulation of illegitimate recombination between two PABLs. © 1996 Oxford University Press Fukagawa Tatsuo Department of Evolutionary Genetics, National Institute of Genetics, and The Graduate University for Advanced Studies, Mishima, Shizuoka-ken 411, Japan aut Nakamura Yasukazu Department of Evolutionary Genetics, National Institute of Genetics, and The Graduate University for Advanced Studies, Mishima, Shizuoka-ken 411, Japan aut Okumura Katsuzumi Faculty of Bioresources, Mie University, Tsu, Mie-ken 514, Japan aut Nogami Masahiro Faculty of Bioresources, Mie University, Tsu, Mie-ken 514, Japan aut Ando Asako School of Medicine, Tokai University, Bohseidai, Isehara, Kanagawa-ken 259-11, Japan aut Inoko Hidetoshi School of Medicine, Tokai University, Bohseidai, Isehara, Kanagawa-ken 259-11, Japan aut Saitou Naruya Department of Evolutionary Genetics, National Institute of Genetics, and The Graduate University for Advanced Studies, Mishima, Shizuoka-ken 411, Japan aut Ikemura Toshimichi Department of Evolutionary Genetics, National Institute of Genetics, and The Graduate University for Advanced Studies, Mishima, Shizuoka-ken 411, Japan aut Human Molecular Genetics Oxford University Press 5/1(1996-01), 23-32 0964-6906 5:1<23 1996 5 hmg https://doi.org/10.1093/hmg/5.1.23 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/hmg/5.1.23 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fukagawa Tatsuo Department of Evolutionary Genetics, National Institute of Genetics, and The Graduate University for Advanced Studies, Mishima, Shizuoka-ken 411, Japan aut NATIONALLICENCE 700 1- Nakamura Yasukazu Department of Evolutionary Genetics, National Institute of Genetics, and The Graduate University for Advanced Studies, Mishima, Shizuoka-ken 411, Japan aut NATIONALLICENCE 700 1- Okumura Katsuzumi Faculty of Bioresources, Mie University, Tsu, Mie-ken 514, Japan aut NATIONALLICENCE 700 1- Nogami Masahiro Faculty of Bioresources, Mie University, Tsu, Mie-ken 514, Japan aut NATIONALLICENCE 700 1- Ando Asako School of Medicine, Tokai University, Bohseidai, Isehara, Kanagawa-ken 259-11, Japan aut NATIONALLICENCE 700 1- Inoko Hidetoshi School of Medicine, Tokai University, Bohseidai, Isehara, Kanagawa-ken 259-11, Japan aut NATIONALLICENCE 700 1- Saitou Naruya Department of Evolutionary Genetics, National Institute of Genetics, and The Graduate University for Advanced Studies, Mishima, Shizuoka-ken 411, Japan aut NATIONALLICENCE 700 1- Ikemura Toshimichi Department of Evolutionary Genetics, National Institute of Genetics, and The Graduate University for Advanced Studies, Mishima, Shizuoka-ken 411, Japan aut NATIONALLICENCE 773 0- Human Molecular Genetics Oxford University Press 5/1(1996-01), 23-32 0964-6906 5:1<23 1996 5 hmg Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford