caa a22 4500 397575475 CHVBK 20180308164854.0 cr unu---uuuuu 161202e199604 xx s 000 0 eng 10.1093/hmg/5.4.451 doi (NATIONALLICENCE)oxford-10.1093/hmg/5.4.451 Xist expression from an Xist YAC transgene carried on the mouse Y chromosome [Elektronische Daten] [S. Matsuura, V. Episkopou, Renata Hamvas, S. D. M. Brown] we have constructed mouse transgenic lines carrying a yac clone encompassing the xist gene in order to investigate the factors influencing Xist expression and the initiation of X-inactivation. two transgenic lines were derived, one carrying four copies integrated at an autosomal site and a second line carrying four copies integrated at a single site on the y chromosome. Xist expression was not observed in mice carrying the autosomal insertion. However, Xist expression from the Y-inserted transgenes was observed and at levels commensurate with that found in normal female mice. methylation sites in the autosomal transgene both 5′ and 3′ of the Xist gene are hypermethylated and appear to reflect methylation patterns observed on the active x chromosome. For the Y-linked transgene, methylation sites 5′ and 3′ of the Xist gene are hypomethylated reflecting patterns found on the inactive X chromosome. however, the 5′ and 3′ methylation levels have been decoupled at the active transgenic locus. the data suggest that sequences in the vicinity of Xist can initiate some of the features that are associated with the initiation process of X-inactivation. © 1996 Oxford University Press Matsuura S. Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, London W2 1PG, UK aut Episkopou V. Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, London W2 1PG, UK aut Hamvas Renata Genome Analysis Laboratory, Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, London WC2A 3PX, UK. aut Brown S. D. M. Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, London W2 1PG, UK aut Human Molecular Genetics Oxford University Press 5/4(1996-04), 451-459 0964-6906 5:4<451 1996 5 hmg https://doi.org/10.1093/hmg/5.4.451 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/hmg/5.4.451 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Matsuura S. Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, London W2 1PG, UK aut NATIONALLICENCE 700 1- Episkopou V. Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, London W2 1PG, UK aut NATIONALLICENCE 700 1- Hamvas Renata Genome Analysis Laboratory, Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, London WC2A 3PX, UK aut NATIONALLICENCE 700 1- Brown S. D. M. Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, London W2 1PG, UK aut NATIONALLICENCE 773 0- Human Molecular Genetics Oxford University Press 5/4(1996-04), 451-459 0964-6906 5:4<451 1996 5 hmg Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford