caa a22 4500 397575696 CHVBK 20180308164855.0 cr unu---uuuuu 161202e199608 xx s 000 0 eng 10.1093/hmg/5.8.1155 doi (NATIONALLICENCE)oxford-10.1093/hmg/5.8.1155 Mouse/Human Sequence Divergence in a Region with a Paternal-Specific Methylation Imprint at the Human H19 Locus [Elektronische Daten] [Yoshihiro Jinno, Kazuo Sengoku, Mitsuyoshi Nakao, Kenichi Tamate, Toshinobu Miyamoto, Tetsuo Matsuzaka, James S. Sutcliffe, Tadashi Anan, Naoyuki Takuma, Kunihiko Nishiwaki, Yuichiro Ikeda, Tadayuki Ishimaru, Mutsuo Ishikawa, Norio Niikawa] We have identified a region with characteristics of a paternal-specific methylation imprint at the human H19 locus. This region, extending from −2.0 kb upstream to the start of transcription, is heavily methylated in sperm and on the paternal allele in somatic cells. This methylation was preserved during pre-implantation. Structural analysis revealed the presence of CpG islands and a large direct repeat with a 400 bp sequence reiterated several times, but no significant sequence homology to the corresponding region of the mouse H19 gene. These findings could suggest a role for secondary DNA structure in genomic imprinting across the species, and they also present a puzzling aspect of the evolution of the H19 regulatory region in human and mouse. © 1996 Oxford University Press Jinno Yoshihiro Department of Human Genetics, Nagasaki University School of Medicine, Nagasaki 852, Japan aut Sengoku Kazuo Department of Obstetrics and Gynecology, Asahikawa Medical College, Asahikawa 078, Japan aut Nakao Mitsuyoshi Department of Tumor Genetics and Biology, Kumamoto University School of Medicine, Kumamoto 860, Japan aut Tamate Kenichi Department of Obstetrics and Gynecology, Asahikawa Medical College, Asahikawa 078, Japan aut Miyamoto Toshinobu Department of Human Genetics, Nagasaki University School of Medicine, Nagasaki 852, Japan aut Matsuzaka Tetsuo Department of Pediatrics, Nagasaki University School of Medicine, Nagasaki 852, Japan aut Sutcliffe James S. Howard Hughes Medical Institute and Department of Molecular and Human Genetics, Baylor College of Medicine, Houston TX77030, USA aut Anan Tadashi Department of Tumor Genetics and Biology, Kumamoto University School of Medicine, Kumamoto 860, Japan aut Takuma Naoyuki Department of Obstetrics and Gynecology, Asahikawa Medical College, Asahikawa 078, Japan aut Nishiwaki Kunihiko Department of Obstetrics and Gynecology, Asahikawa Medical College, Asahikawa 078, Japan aut Ikeda Yuichiro Department of Obstetrics and Gynecology, Nagasaki University School of Medicine, Nagasaki 852, Japan aut Ishimaru Tadayuki Department of Obstetrics and Gynecology, Nagasaki University School of Medicine, Nagasaki 852, Japan aut Ishikawa Mutsuo Department of Obstetrics and Gynecology, Asahikawa Medical College, Asahikawa 078, Japan aut Niikawa Norio Department of Human Genetics, Nagasaki University School of Medicine, Nagasaki 852, Japan aut Human Molecular Genetics Oxford University Press 5/8(1996-08), 1155-1161 0964-6906 5:8<1155 1996 5 hmg https://doi.org/10.1093/hmg/5.8.1155 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/hmg/5.8.1155 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jinno Yoshihiro Department of Human Genetics, Nagasaki University School of Medicine, Nagasaki 852, Japan aut NATIONALLICENCE 700 1- Sengoku Kazuo Department of Obstetrics and Gynecology, Asahikawa Medical College, Asahikawa 078, Japan aut NATIONALLICENCE 700 1- Nakao Mitsuyoshi Department of Tumor Genetics and Biology, Kumamoto University School of Medicine, Kumamoto 860, Japan aut NATIONALLICENCE 700 1- Tamate Kenichi Department of Obstetrics and Gynecology, Asahikawa Medical College, Asahikawa 078, Japan aut NATIONALLICENCE 700 1- Miyamoto Toshinobu Department of Human Genetics, Nagasaki University School of Medicine, Nagasaki 852, Japan aut NATIONALLICENCE 700 1- Matsuzaka Tetsuo Department of Pediatrics, Nagasaki University School of Medicine, Nagasaki 852, Japan aut NATIONALLICENCE 700 1- Sutcliffe James S. Howard Hughes Medical Institute and Department of Molecular and Human Genetics, Baylor College of Medicine, Houston TX77030, USA aut NATIONALLICENCE 700 1- Anan Tadashi Department of Tumor Genetics and Biology, Kumamoto University School of Medicine, Kumamoto 860, Japan aut NATIONALLICENCE 700 1- Takuma Naoyuki Department of Obstetrics and Gynecology, Asahikawa Medical College, Asahikawa 078, Japan aut NATIONALLICENCE 700 1- Nishiwaki Kunihiko Department of Obstetrics and Gynecology, Asahikawa Medical College, Asahikawa 078, Japan aut NATIONALLICENCE 700 1- Ikeda Yuichiro Department of Obstetrics and Gynecology, Nagasaki University School of Medicine, Nagasaki 852, Japan aut NATIONALLICENCE 700 1- Ishimaru Tadayuki Department of Obstetrics and Gynecology, Nagasaki University School of Medicine, Nagasaki 852, Japan aut NATIONALLICENCE 700 1- Ishikawa Mutsuo Department of Obstetrics and Gynecology, Asahikawa Medical College, Asahikawa 078, Japan aut NATIONALLICENCE 700 1- Niikawa Norio Department of Human Genetics, Nagasaki University School of Medicine, Nagasaki 852, Japan aut NATIONALLICENCE 773 0- Human Molecular Genetics Oxford University Press 5/8(1996-08), 1155-1161 0964-6906 5:8<1155 1996 5 hmg Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford