caa a22 4500 397575793 CHVBK 20180308164856.0 cr unu---uuuuu 161202e199611 xx s 000 0 eng 10.1093/hmg/5.11.1801 doi (NATIONALLICENCE)oxford-10.1093/hmg/5.11.1801 Testis-Specific Protein, Y-Encoded (TSPY) Expression in Testicular Tissues [Elektronische Daten] [Frank Schnieders, Thilo Dörk, Joachim Arnemann, Tanja Vogel, Martin Werner, Jörg Schmidtke] TSPY, the ‘testis-specific protein, Y-encoded', is the product of a tandem gene cluster on human proximal Yp. In order to gain insight into the function of this locus, we have analysed (i) the diversity of RNAs transcribed from the cluster, (ii) the sequence homology of the deduced TSPY to other proteins, and (iii) its protein properties both in tissue extracts and in tissue sections, using a TSPY-specific antiserum. We have identified a set of distinct TSPY transcripts with diverse exon compositions. We show that TSPY has homology with other human and non-human proteins, including SET and NAP, factors that are suggested to play a role in DNA replication. Protein analysis revealed TSPY to occur mainly in a modified, putatively phosphorylated form. By immunostaining it was detected in distinct subsets of spermatogonia. TSPY was also strongly immunostained in early testicular carcinoma in situ (CIS), while seminomatous tumour cells stained less intensely. The spermatogonial cells of two XY-TFM-females gave a strong immune response. The data presented here point to a phosphorylation-dependent TSPY-function in early spermatogenesis, immediately prior to the spermatogonia-to-spermatocyte transition, and in early testicular tumorigenesis. © 1996 Oxford University Press Schnieders Frank Institut für Humangenetik, Medizinische Hochschule Hannover, 30623 Hannover, Germany aut Dörk Thilo Institut für Humangenetik, Medizinische Hochschule Hannover, 30623 Hannover, Germany aut Arnemann Joachim Institut für Humangenetik, Johann-Wolfgang-Goethe-Universität zu Frankfurt, 60590 Frankfurt, Germany aut Vogel Tanja Institut für Humangenetik, Medizinische Hochschule Hannover, 30623 Hannover, Germany aut Werner Martin Abteilung für Experimentelle Pathologie, Medizinische Hochschule Hannover, 30623 Hannover, Germany aut Schmidtke Jörg Institut für Humangenetik, Medizinische Hochschule Hannover, 30623 Hannover, Germany aut Human Molecular Genetics Oxford University Press 5/11(1996-11), 1801-1807 0964-6906 5:11<1801 1996 5 hmg https://doi.org/10.1093/hmg/5.11.1801 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/hmg/5.11.1801 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schnieders Frank Institut für Humangenetik, Medizinische Hochschule Hannover, 30623 Hannover, Germany aut NATIONALLICENCE 700 1- Dörk Thilo Institut für Humangenetik, Medizinische Hochschule Hannover, 30623 Hannover, Germany aut NATIONALLICENCE 700 1- Arnemann Joachim Institut für Humangenetik, Johann-Wolfgang-Goethe-Universität zu Frankfurt, 60590 Frankfurt, Germany aut NATIONALLICENCE 700 1- Vogel Tanja Institut für Humangenetik, Medizinische Hochschule Hannover, 30623 Hannover, Germany aut NATIONALLICENCE 700 1- Werner Martin Abteilung für Experimentelle Pathologie, Medizinische Hochschule Hannover, 30623 Hannover, Germany aut NATIONALLICENCE 700 1- Schmidtke Jörg Institut für Humangenetik, Medizinische Hochschule Hannover, 30623 Hannover, Germany aut NATIONALLICENCE 773 0- Human Molecular Genetics Oxford University Press 5/11(1996-11), 1801-1807 0964-6906 5:11<1801 1996 5 hmg Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford