caa a22 4500 397590318 CHVBK 20180308164936.0 cr unu---uuuuu 161202e199609 xx s 000 0 eng 10.1093/jac/38.3.399 doi (NATIONALLICENCE)oxford-10.1093/jac/38.3.399 In-vitro activity of macrolides alone and in combination with artemisin, atovaquone, dapsone, minocycline or pyrimethamine against Cryptosporidium parvum [Elektronische Daten] [Andrea Giacometti, Oscar Cirioni, Giorgio Scalise] The anticryptosporidial activity of four macrolides alone and in combination with other antimicrobial agents was investigated against ten clinical isolates of Cryptosporidium parvum recovered from stools of AIDS patients. The susceptibility tests were performed by inoculation of the protozoa on to cell monolayers and determining the parasite count after 72 h incubation at 37°C. The culture medium was supplemented with Dulbecco's modified Eagle's medium containing serial dilutions of azithromycin, clarithromycin, roxithromycin, spiramycin, alone or in combination with artemisin, atovaquone, dapsone, minocycline or pyrimethamine. Most of the agents had an inhibitory effect on parasite growth, but only at high concentrations. No agent was able to inhibit parasite growth completely, even at the highest concentrations used. The more effective agents, azithromycin, clarithromycin, roxithromycin, minocycline and pyrimethamine, produced no more than a 13.1-27.8% reduction in oocyst count and no more than a 15.1-35.7% in schizont count. Positive interaction was clearly demonstrated when macrolides were tested in combination with minocycline or pyrimethamine. © 1996 The British Society for Antimicrobial Chemotherapy Original articles nationallicence Giacometti Andrea Institute of Infectious Diseases and Public Health, University of Ancona, Italy aut Cirioni Oscar Institute of Infectious Diseases and Public Health, University of Ancona, Italy aut Scalise Giorgio Institute of Infectious Diseases and Public Health, University of Ancona, Italy aut Journal of Antimicrobial Chemotherapy Oxford University Press 38/3(1996-09), 399-408 0305-7453 38:3<399 1996 38 jac https://doi.org/10.1093/jac/38.3.399 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/38.3.399 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Giacometti Andrea Institute of Infectious Diseases and Public Health, University of Ancona, Italy aut NATIONALLICENCE 700 1- Cirioni Oscar Institute of Infectious Diseases and Public Health, University of Ancona, Italy aut NATIONALLICENCE 700 1- Scalise Giorgio Institute of Infectious Diseases and Public Health, University of Ancona, Italy aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 38/3(1996-09), 399-408 0305-7453 38:3<399 1996 38 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford