caa a22 4500 397590407 CHVBK 20180308164936.0 cr unu---uuuuu 161202e199610 xx s 000 0 eng 10.1093/jac/38.4.599 doi (NATIONALLICENCE)oxford-10.1093/jac/38.4.599 The elution and binding characteristics of rifampicin for three commercially available protein-sealed vascular grafts [Elektronische Daten] [A. M. Lovering, L. O. White, A. P. MacGowan, D. S. Reeves] Rifampicin was absorbed onto gelatin-sealed (Gelsoft and Unigraft) or collagen-sealed (Hemashield) vascular grafts by soaking for 15 min in a 1000 mg/L solution. Bound drug was then eluted from the grafts by incubation in phosphate buffered saline (PBS) at 37°C and at timed intervals the concentration of rifampicin remaining in the grafts was determined. Although all three grafts contained high concentrations of rifampicin immediately after absorption of drug, rifampicin concentrations rapidly fell during elution with PBS to approximately 1.25 mg/kg of graft after 5 h incubation in PBS, indicating that most of the rifampicin absorbed to the grafts was only loosely bound. However, once this loosely bound fraction had been removed there was a much slower elution of the remaining rifampicin from the grafts, suggesting a second and much more tightly bound fraction. The tightly bound fraction eluted with an apparent half-life of 47-76 h, depending on the graft, and extrapolation back to time zero from this phase suggests that only a very small amount of the rifampicin is tightly bound to the graft after initial soaking (0.6-1.3 mg/kg). © 1995 The British Society for Antimicrobial Chemotherapy Original articles nationallicence Lovering A. M. Bristol Centre for Antimicrobial Research and Evaluation, Department of Medical Microbiology, Southmead Hospital, Westbury on Trym, Bristol BS10 5NB, UK aut White L. O. Bristol Centre for Antimicrobial Research and Evaluation, Department of Medical Microbiology, Southmead Hospital, Westbury on Trym, Bristol BS10 5NB, UK aut MacGowan A. P. Bristol Centre for Antimicrobial Research and Evaluation, Department of Medical Microbiology, Southmead Hospital, Westbury on Trym, Bristol BS10 5NB, UK aut Reeves D. S. Bristol Centre for Antimicrobial Research and Evaluation, Department of Medical Microbiology, Southmead Hospital, Westbury on Trym, Bristol BS10 5NB, UK aut Journal of Antimicrobial Chemotherapy Oxford University Press 38/4(1996-10), 599-604 0305-7453 38:4<599 1996 38 jac https://doi.org/10.1093/jac/38.4.599 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/38.4.599 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lovering A. M. Bristol Centre for Antimicrobial Research and Evaluation, Department of Medical Microbiology, Southmead Hospital, Westbury on Trym, Bristol BS10 5NB, UK aut NATIONALLICENCE 700 1- White L. O. Bristol Centre for Antimicrobial Research and Evaluation, Department of Medical Microbiology, Southmead Hospital, Westbury on Trym, Bristol BS10 5NB, UK aut NATIONALLICENCE 700 1- MacGowan A. P. Bristol Centre for Antimicrobial Research and Evaluation, Department of Medical Microbiology, Southmead Hospital, Westbury on Trym, Bristol BS10 5NB, UK aut NATIONALLICENCE 700 1- Reeves D. S. Bristol Centre for Antimicrobial Research and Evaluation, Department of Medical Microbiology, Southmead Hospital, Westbury on Trym, Bristol BS10 5NB, UK aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 38/4(1996-10), 599-604 0305-7453 38:4<599 1996 38 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford