caa a22 4500 397590415 CHVBK 20180308164937.0 cr unu---uuuuu 161202e199607 xx s 000 0 eng 10.1093/jac/38.1.133 doi (NATIONALLICENCE)oxford-10.1093/jac/38.1.133 A mechanism of rifamycin inhibition and resistance in Pseudomonas aeruginosa [Elektronische Daten] [Ying Cheung Yee, Benjamin Kisslinger, Victor L. Yu, Ding Jun Jin] We sought to study the nature of rifampicin resistance in Pseudomonas aeruginosa. We hypothesized that the rifamycin regions of RNA polymerase are conserved in P. aeruginosa and that rifampicin resistance is mediated by a mutation in the rpoB gene encoding the β subunit of RNA polymerase. Transcription assays showed that 50 nM of rifampicin inhibited transcription > 99% in a clinical isolate (MIC = 32 mg/L) and only < 40% in the rifampicin resistant mutant (MIC = 1000 mg/L). DNA sequencing revealed that the rifampicin regions are conserved in P. aeruginosa and the rifampicin regions of the rifampicin-resistant strain contained a mutation. Sodium hexametaphosphate lowered rifamycin MIC in a rifamycin-resistant mutant four-fold and in the clinical isolate 32-fold, suggesting that P. aeruginosa has a natural membrane barrier to rifamycins. © 1996 by the British Society for Antimicrobial Chemotherapy Brief reports nationallicence Yee Ying Cheung University of Pittsburgh Graduate School of Public Health, Department of Infectious Diseases and Microbiology Pittsburgh, Pennsylvania 15261 aut Kisslinger Benjamin Carnegie Mellon University, Mellon Institute 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213 aut Yu Victor L. University of Pittsburgh Graduate School of Public Health, Department of Infectious Diseases and Microbiology Pittsburgh, Pennsylvania 15261 aut Jin Ding Jun Laboratory of Molecular Biology National Cancer Institute, National Institutes of Health, Bethesda Maryland 20892-4255, USA aut Journal of Antimicrobial Chemotherapy Oxford University Press 38/1(1996-07), 133-137 0305-7453 38:1<133 1996 38 jac https://doi.org/10.1093/jac/38.1.133 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/38.1.133 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yee Ying Cheung University of Pittsburgh Graduate School of Public Health, Department of Infectious Diseases and Microbiology Pittsburgh, Pennsylvania 15261 aut NATIONALLICENCE 700 1- Kisslinger Benjamin Carnegie Mellon University, Mellon Institute 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213 aut NATIONALLICENCE 700 1- Yu Victor L. University of Pittsburgh Graduate School of Public Health, Department of Infectious Diseases and Microbiology Pittsburgh, Pennsylvania 15261 aut NATIONALLICENCE 700 1- Jin Ding Jun Laboratory of Molecular Biology National Cancer Institute, National Institutes of Health, Bethesda Maryland 20892-4255, USA aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 38/1(1996-07), 133-137 0305-7453 38:1<133 1996 38 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford