caa a22 4500 397590482 CHVBK 20180308164937.0 cr unu---uuuuu 161202e199612 xx s 000 0 eng 10.1093/jac/38.6.1031 doi (NATIONALLICENCE)oxford-10.1093/jac/38.6.1031 Resistance to bismuth among Gram-negative bacteria is dependent upon iron and its uptake [Elektronische Daten] [Philip Domenico, Jay Reich, William Madonia, Burke A. Cunha] Bismuth antimicrobial action is poorly understood. Many tnvalent metals possess antibacterial activity, especially under low iron conditions. Protection of bacteria from the deleterious effects of bismuth and other trivalent metals was demonstrated in iron-fortified media. Near-equimolar quantities of Fe3+ neutralized the growth-inhibitory effects of 250 μm Bi3+. Resistance to bismuth action also depended on the production of virulence-related siderophores. Escherichia coli, Aeromonas hydrophila or Pseudomonas aeruginosa producing aerobactin, amonabactin or pyoverdin respectively, were most resistant to Bi3+. Enterochelin or pyochelin producers were less resistant to Bi3+, but more resistant than strains lacking siderophores. Purified pyoverdin restored Bi3+ resistance in a mutant lacking this siderophore, but not in one lacking the pyoverdin receptor. Bismuth-treated bacteria exhibited unique outer membrane proteins, similar in size to iron-repressible proteins. Thus, resistance to the inhibitory action of Bi3+ among Gram-negative bacteria is inversely related to iron concentration and strongly dependent on iron transport mechanisms. The data suggest that bismuth action is largely a nonspecific, competitive interference with iron transport, related primarily to atomic valence. Furthermore, resistance to Bi3+ among bacteria is predictive of virulence. © 1996 by the British Society for Antimicrobial Chemotherapy Original articles nationallicence Domenico Philip Infectious Disease Division, Winthrop-University Hospital Mineola, Long Island, New York 11501 aut Reich Jay Infectious Disease Division, Winthrop-University Hospital Mineola, Long Island, New York 11501 aut Madonia William Infectious Disease Division, Winthrop-University Hospital Mineola, Long Island, New York 11501 aut Cunha Burke A. SUNY School of Medicine Stony Brook, NY, USA aut Journal of Antimicrobial Chemotherapy Oxford University Press 38/6(1996-12), 1031-1040 0305-7453 38:6<1031 1996 38 jac https://doi.org/10.1093/jac/38.6.1031 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/38.6.1031 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Domenico Philip Infectious Disease Division, Winthrop-University Hospital Mineola, Long Island, New York 11501 aut NATIONALLICENCE 700 1- Reich Jay Infectious Disease Division, Winthrop-University Hospital Mineola, Long Island, New York 11501 aut NATIONALLICENCE 700 1- Madonia William Infectious Disease Division, Winthrop-University Hospital Mineola, Long Island, New York 11501 aut NATIONALLICENCE 700 1- Cunha Burke A. SUNY School of Medicine Stony Brook, NY, USA aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 38/6(1996-12), 1031-1040 0305-7453 38:6<1031 1996 38 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford